CAJ | 학술논문

以海岛棉标准系3-79为对照,陆地棉标准系TM-1为参考对照,用SSR引物对来自前苏联、中国、美国和埃及等4个海岛棉主要生产国(地区)的20份海岛棉种质资源的基因组DNA进行SSR分析,研究不同海岛棉生产国的海岛棉品种资源的遗传亲缘关系和遗传多样性.108对SSR引物共获得了175条多态性谱带,平均每个引物扩增出1.62条多态性谱带.供试海岛棉品种之间的遗传相似系数为0.66～0.94.平均值为0.81.根据UPGMA聚类分析,以遗传相似系数阀值为0.77,可将20份栽培棉花种质材料分为4大类:第一类均为前苏联品种,第二类均为中国品种,第三类以美国品种为主,第四类以埃及品种为主.遗传多样性分析结果表明,前苏联海岛棉品种资源的遗传多样性最为丰富,而埃及的海岛棉品种资源遗传距离最狭窄,我国的海岛棉品种资源的遗传多样性居中.本研究表明,供试材料遗传背景与其产地背景有一定关联性.SSR标记能较好地揭示供试棉花品种之间的遗传差异和亲缘关系.
이해도면표준계3-79위대조,륙지면표준계TM-1위삼고대조,용SSR인물대래자전소련、중국、미국화애급등4개해도면주요생산국(지구)적20빈해도면충질자원적기인조DNA진행SSR분석,연구불동해도면생산국적해도면품충자원적유전친연관계화유전다양성.108대SSR인물공획득료175조다태성보대,평균매개인물확증출1.62조다태성보대.공시해도면품충지간적유전상사계수위0.66～0.94.평균치위0.81.근거UPGMA취류분석,이유전상사계수벌치위0.77,가장20빈재배면화충질재료분위4대류:제일류균위전소련품충,제이류균위중국품충,제삼류이미국품충위주,제사류이애급품충위주.유전다양성분석결과표명,전소련해도면품충자원적유전다양성최위봉부,이애급적해도면품충자원유전거리최협착,아국적해도면품충자원적유전다양성거중.본연구표명,공시재료유전배경여기산지배경유일정관련성.SSR표기능교호지게시공시면화품충지간적유전차이화친연관계.
Gemomic DNA of 20 germplasms of sea island cotton (G.barbadense L.) from four production countries(regions),namely former USSR, China, USA, and Egypt, were analyzed for their genetic relationship and genetic diversity, using the 3-78(the standard line of G.barbadeuse) as check and TM-1 (the standard line of G.hirsutum) as reference check. The SSR molecular markers were employed and 108 pairs of polymorphic SSR primers were used in this experiment. Among the 108 pairs of polymorphic SSR primers, 175 polymorphic bands were produced, with averaging of 1.62 bands per primer. The genetic similarity coefficient among the tested germplasms was 0.66-0.94, with the average of 0.81. The UPGMA cluster analysis showed that four groups could be clearly clustered when genetic similarity coefficient was given as 0.77. The first group were those from formerUSSR, most germplasms in the second group came from USA, all of the germplasms in the third group came from China, and most in the last group were those from Egypt. The results showed that the genetic diversity of the sea-island cotton germpalsms from USSR was extraordinary diversified among the four different countries, and that from Egypt was the least in genetic diversity. The sea-island cotton germplasms from China were placed in the middle, according to the results of genetic similarity coefficient in present experiment. Our results also showed that the genetic background of the sea-island cotton germplasms tested inthis experiment was correlative with their producing regions, and the SSR marker could be used in revealing the genetic diversity and genetic relationship among the sea-island germplasms.