CAJ | 학술논문

目的应用基质细胞衍生因子(SDF-1)结合外周血内皮祖细胞(EPCs)移植治疗裸鼠肢体缺血,探讨其促进血管新生的能力.方法建立裸鼠后肢缺血模型,随机分为缺血对照组、EPCs移植组、SDF-1组、SDF-1联合EPCs移植组及SDF-1联合AMD3100处理后的EPCs移植组.移植第3、7天检测后肢腓肠肌CD34+VEGFR+细胞数,第28天检测缺血区新生血管密度.结果移植第3天对照组、EPCs组、SDF-1组、SDF-1+EPCs组、SDF-1+AMD3100 EPCs组双阳性细胞数分别为0.00±0.00个/HP、5.30±0.65个/HP、0.00±0.00个/HP、10.31±0.63个/HP及1.86±0.17个/HP;第7天分别为0.00±0.00个/HP、7.05±0.18个/HP、0.00±0.00个/HP、11.81±0.53个/HP及2.83±0.48个/HP,第28天新生毛细血管数分别为3.00 ±0.13个/HP、6.15±0.04个/HP、6.20±0.10个/HP、10.65 ±0.08个/HP、6.21±0.08个/HP.SDF-1可增加缺血区CD34+ VEGFR+细胞浸润数量(P＜0.05),提高新生血管数量(P＜0.05),SDF-1联合外周血EPCs可进一步提高新生血管数量(P＜0.05).结论 SDF-1能够促进EPCs归巢,增强血管形成能力,促进血管新生;AMD3100能够阻断SDF-1的诱导迁移效应.
목적 응용기질세포연생인자(SDF-1)결합외주혈내피조세포(EPCs)이식치료라서지체결혈,탐토기촉진혈관신생적능력.방법 건립라서후지결혈모형,수궤분위결혈대조조、EPCs이식조、SDF-1조、SDF-1연합EPCs이식조급SDF-1연합AMD3100처리후적EPCs이식조.이식제3、7천검측후지비장기CD34+VEGFR+세포수,제28천검측결혈구신생혈관밀도.결과 이식제3천대조조、EPCs조、SDF-1조、SDF-1+EPCs조、SDF-1+AMD3100 EPCs조쌍양성세포수분별위0.00±0.00개/HP、5.30±0.65개/HP、0.00±0.00개/HP、10.31±0.63개/HP급1.86±0.17개/HP;제7천분별위0.00±0.00개/HP、7.05±0.18개/HP、0.00±0.00개/HP、11.81±0.53개/HP급2.83±0.48개/HP,제28천신생모세혈관수분별위3.00 ±0.13개/HP、6.15±0.04개/HP、6.20±0.10개/HP、10.65 ±0.08개/HP、6.21±0.08개/HP.SDF-1가증가결혈구CD34+ VEGFR+세포침윤수량(P＜0.05),제고신생혈관수량(P＜0.05),SDF-1연합외주혈EPCs가진일보제고신생혈관수량(P＜0.05).결론 SDF-1능구촉진EPCs귀소,증강혈관형성능력,촉진혈관신생;AMD3100능구조단SDF-1적유도천이효응.
Objective To explore the effect of stromal cell-derived factor-1 ( SDF-1 ) in combination with transplantation of peripheral blood endothelial progenitor cells (EPCs) for the treatment of nude mice hindlimb ischemia. Methods Hindlimb ischemia model was established in nude mice, mice were then divided into five groups randomly: ischemic control group, peripheral blood EPCs transplantation group, SDF-1 local application group, SDF-1 combined with EPCs group, SDF-1 combined with AMD3100 treated EPCs group. Local CD34+VEGFR+ cells in the hind gastrocnemius were detected at day 3,7 after transplantation. The intensity of neovasculorization were evaluated at day 28. Results The double-positive cells number of control group, EPCs group, SDF-1 group, SDF-1 + EPCs group, SDF-1 + AMD3100 EPCs group were 0.00 ±0.00,5. 30 ±0.65,0.00 ±0.00,10. 31 ±0. 63,1. 86 ±0. 17 at day 3 and 0. 00 ±0. 00, 7.05 ±0. 18,0. 00 ±0. 00,11. 81 ±0. 53,2. 83 ±0. 48 at day 7. The number of new capillaries were 3. 00 ± 0.13,6.15 ± 0. 04,6. 20 ± 0. 10,10. 65 ± 0.08,6. 21 ±0. 08 at day 28. SDF-1 increased the CD34 + VEGFR+ cells (P ＜0. 05) and the number of new vessels (P ＜0.05). SDF-1 combined EPCs further increased the number of new vessels (P ＜ 0. 05 ). Conclusions SDF-1 enhances blood vessel formation and promotes angiogenesis by promoting EPCs homing, which could be blocked by AMD3100.