中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
3期
308-311
,共4页
申恩华%王立红%王辉%孙宏莉%陈民钧%袁静%王彦宽
申恩華%王立紅%王輝%孫宏莉%陳民鈞%袁靜%王彥寬
신은화%왕립홍%왕휘%손굉리%진민균%원정%왕언관
金黄色葡萄球菌%耐甲氧西林%多位点序列分型%葡萄球菌染色体mec分型%葡萄球菌A蛋白分型
金黃色葡萄毬菌%耐甲氧西林%多位點序列分型%葡萄毬菌染色體mec分型%葡萄毬菌A蛋白分型
금황색포도구균%내갑양서림%다위점서렬분형%포도구균염색체mec분형%포도구균A단백분형
Methicillin-resistant Staphylococcus aureus%Multilocus sequence typing%Staphylococcal cassette chromosome mec%Staphylococcus protein A typing
目的 研究中国2006年耐甲氧西林金黄色葡萄球菌(MRSA)流行株的来源和遗传背景.方法 收集2006年1-12月15个地区17家医院连续分离的非重复MRSA 302株,通过多重PCR对MRSA进行染色体mec(SCCmec)分型,葡萄球菌A蛋白(Spa)分型和多位点序列分型(MLST).结果 SCCmec分型Ⅲ型为76.8%,不能分型为7.9%.从广州地区分离株中发现2株Ⅳ型,Ⅱ型为14.6%;而大连地区分离株中Ⅱ型为75.0%,与其他地区菌株分型的差异存在统计学意义(P<0.005).MLST共有4种分型,其中序列分型(ST)239(46.7%)、ST5(44.4%)、ST59(6.7%)、ST88(2.2%).Spa分型共有14种,其中t30(52.6%)、t37(27.2%)、t2(12.9%)、t632(2.3%)、t437(1.3%)和t570、t601(各0.7%)及t377、t459、t796、t899、t1152、t2649(各0.3%),未分型占0.3%.未检出pvl以基因.结论 调查的15个地区MRSA主要克隆株为ST239-MRSA-SCCmecⅢ-t30和ST5-MRSA-SCCmecⅡ-t2,具有独特的地理分布.
目的 研究中國2006年耐甲氧西林金黃色葡萄毬菌(MRSA)流行株的來源和遺傳揹景.方法 收集2006年1-12月15箇地區17傢醫院連續分離的非重複MRSA 302株,通過多重PCR對MRSA進行染色體mec(SCCmec)分型,葡萄毬菌A蛋白(Spa)分型和多位點序列分型(MLST).結果 SCCmec分型Ⅲ型為76.8%,不能分型為7.9%.從廣州地區分離株中髮現2株Ⅳ型,Ⅱ型為14.6%;而大連地區分離株中Ⅱ型為75.0%,與其他地區菌株分型的差異存在統計學意義(P<0.005).MLST共有4種分型,其中序列分型(ST)239(46.7%)、ST5(44.4%)、ST59(6.7%)、ST88(2.2%).Spa分型共有14種,其中t30(52.6%)、t37(27.2%)、t2(12.9%)、t632(2.3%)、t437(1.3%)和t570、t601(各0.7%)及t377、t459、t796、t899、t1152、t2649(各0.3%),未分型佔0.3%.未檢齣pvl以基因.結論 調查的15箇地區MRSA主要剋隆株為ST239-MRSA-SCCmecⅢ-t30和ST5-MRSA-SCCmecⅡ-t2,具有獨特的地理分佈.
목적 연구중국2006년내갑양서림금황색포도구균(MRSA)류행주적래원화유전배경.방법 수집2006년1-12월15개지구17가의원련속분리적비중복MRSA 302주,통과다중PCR대MRSA진행염색체mec(SCCmec)분형,포도구균A단백(Spa)분형화다위점서렬분형(MLST).결과 SCCmec분형Ⅲ형위76.8%,불능분형위7.9%.종엄주지구분리주중발현2주Ⅳ형,Ⅱ형위14.6%;이대련지구분리주중Ⅱ형위75.0%,여기타지구균주분형적차이존재통계학의의(P<0.005).MLST공유4충분형,기중서렬분형(ST)239(46.7%)、ST5(44.4%)、ST59(6.7%)、ST88(2.2%).Spa분형공유14충,기중t30(52.6%)、t37(27.2%)、t2(12.9%)、t632(2.3%)、t437(1.3%)화t570、t601(각0.7%)급t377、t459、t796、t899、t1152、t2649(각0.3%),미분형점0.3%.미검출pvl이기인.결론 조사적15개지구MRSA주요극륭주위ST239-MRSA-SCCmecⅢ-t30화ST5-MRSA-SCCmecⅡ-t2,구유독특적지리분포.
Objective To investigate the source and genetic background of methicillin-resistant Staphylococcus aureus in the year of 2006,in China. Methods From January to December 2006,a total number of 302 consecutive and non-repetitive methicillin-resistant Staphylococcus aureus were collected from 17 Teaching hospitals in 15 areas. Genotypes of SCCmec were determined by multiplex PCR and multilocus sequence typing (MLST) was used to type the house-keeping genes. The implementation of the spa typing method was straightforward,and the results obtained were reproducible,unambiguous,and easily interpreted. Results All areas but Dalian harbored SCCmec Ⅲ while Dalian harbored SCCmec Ⅱ most. There were two strains in Guangzhou,harboring SCCmec Ⅳ. There were four strains of sequence type(ST),with ST239 accounted for 46.7% and ST5 accounted for 44.4%. ST59 accounted for 6.7% and ST88 accounted for 2.2%. There were fourteen strains of Spa typing,with t30 accounted for 52.6% ; t37 accounted for 27.2% ; t2 accounted for 12.9% ; t632 accounted for 2.3% ; t437 accounted for 1.3% ; t570,t601 accounted for 0.7% ; t377,t459,t796,t899,t1152,t2649 accounted for 0.3% ; no-typing accounted for 0.3%,respectively,pvl gene was not detected. Conclusion The main clone strains were ST239-MRSA-SCCmec Ⅲ-t30,ST5-MRSA-SCCmec Ⅱ-t2,with unique geographic distributions across the whole nation.
