中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
6期
413-416
,共4页
齐莹%石磊%高岚岳%王高阳%李革新%吕秀强%金亚平
齊瑩%石磊%高嵐嶽%王高暘%李革新%呂秀彊%金亞平
제형%석뢰%고람악%왕고양%리혁신%려수강%금아평
1%2-二氯乙烷%小鼠%旷场实验%神经递质
1%2-二氯乙烷%小鼠%曠場實驗%神經遞質
1%2-이록을완%소서%광장실험%신경체질
1,2-dichloroethane%Mice%Open field test%Neurotransmitter
目的 探讨亚急性1,2-二氯乙烷(1,2-dichloroethane,1,2-DCE)染毒对小鼠行为及脑神经递质含量的影响,为揭示1,2-DCE神经毒性机制提供参考.方法 将昆明种小鼠随机分为4组:对照组和低、中、高剂量1,2-DCE染毒组(225、450、900 mg/m3),每组8只小鼠,静式吸入染毒10 d,每天染毒3.5 h.最后一次染毒结束后立即进行旷场试验,实验结束后处死小鼠,快速取大脑组织,采用高效液相色谱法(HPLC)检测脑组织中谷氨酸(Glu)、天冬氨酸(Asp)及γ-氨基丁酸(GABA)的含量.结果 各染毒组小鼠脑组织中Asp和Glu含量随染毒剂量的增加而升高,且各染毒组小鼠的Glu含量[低、中、高剂量染毒组分别为(67.69±9.89)、(67.99±6.23)、(71.16±5.96)μmol/g Pro]与对照组[(50.78±5.15)μmol/g Pro]比较,差异均有统计学意义(P<0.01);低剂量染毒组小鼠脑组织中GABA含量[(8.08±2.37)μmol/g Pro]较对照组[(12.83±3.36)μmol/g Pro]明显降低,但高剂量组[(19.87±5.30)μmol/g Pro]与对照组比较,明显升高,差异均有统计学意义(P<0.05,P<0.01).低剂量1,2-DCE染毒对小鼠的行为有兴奋作用;而高剂量1,2-DCE染毒对小鼠的探索性及运动性行为有抑制作用.结论 亚急性1,2-DCE染毒可引起小鼠脑组织中氨基酸类神经递质含量及比值的变化,进而导致出现行为的改变,这可能是1,2-DCE神经毒性作用的机制之一.
目的 探討亞急性1,2-二氯乙烷(1,2-dichloroethane,1,2-DCE)染毒對小鼠行為及腦神經遞質含量的影響,為揭示1,2-DCE神經毒性機製提供參攷.方法 將昆明種小鼠隨機分為4組:對照組和低、中、高劑量1,2-DCE染毒組(225、450、900 mg/m3),每組8隻小鼠,靜式吸入染毒10 d,每天染毒3.5 h.最後一次染毒結束後立即進行曠場試驗,實驗結束後處死小鼠,快速取大腦組織,採用高效液相色譜法(HPLC)檢測腦組織中穀氨痠(Glu)、天鼕氨痠(Asp)及γ-氨基丁痠(GABA)的含量.結果 各染毒組小鼠腦組織中Asp和Glu含量隨染毒劑量的增加而升高,且各染毒組小鼠的Glu含量[低、中、高劑量染毒組分彆為(67.69±9.89)、(67.99±6.23)、(71.16±5.96)μmol/g Pro]與對照組[(50.78±5.15)μmol/g Pro]比較,差異均有統計學意義(P<0.01);低劑量染毒組小鼠腦組織中GABA含量[(8.08±2.37)μmol/g Pro]較對照組[(12.83±3.36)μmol/g Pro]明顯降低,但高劑量組[(19.87±5.30)μmol/g Pro]與對照組比較,明顯升高,差異均有統計學意義(P<0.05,P<0.01).低劑量1,2-DCE染毒對小鼠的行為有興奮作用;而高劑量1,2-DCE染毒對小鼠的探索性及運動性行為有抑製作用.結論 亞急性1,2-DCE染毒可引起小鼠腦組織中氨基痠類神經遞質含量及比值的變化,進而導緻齣現行為的改變,這可能是1,2-DCE神經毒性作用的機製之一.
목적 탐토아급성1,2-이록을완(1,2-dichloroethane,1,2-DCE)염독대소서행위급뇌신경체질함량적영향,위게시1,2-DCE신경독성궤제제공삼고.방법 장곤명충소서수궤분위4조:대조조화저、중、고제량1,2-DCE염독조(225、450、900 mg/m3),매조8지소서,정식흡입염독10 d,매천염독3.5 h.최후일차염독결속후립즉진행광장시험,실험결속후처사소서,쾌속취대뇌조직,채용고효액상색보법(HPLC)검측뇌조직중곡안산(Glu)、천동안산(Asp)급γ-안기정산(GABA)적함량.결과 각염독조소서뇌조직중Asp화Glu함량수염독제량적증가이승고,차각염독조소서적Glu함량[저、중、고제량염독조분별위(67.69±9.89)、(67.99±6.23)、(71.16±5.96)μmol/g Pro]여대조조[(50.78±5.15)μmol/g Pro]비교,차이균유통계학의의(P<0.01);저제량염독조소서뇌조직중GABA함량[(8.08±2.37)μmol/g Pro]교대조조[(12.83±3.36)μmol/g Pro]명현강저,단고제량조[(19.87±5.30)μmol/g Pro]여대조조비교,명현승고,차이균유통계학의의(P<0.05,P<0.01).저제량1,2-DCE염독대소서적행위유흥강작용;이고제량1,2-DCE염독대소서적탐색성급운동성행위유억제작용.결론 아급성1,2-DCE염독가인기소서뇌조직중안기산류신경체질함량급비치적변화,진이도치출현행위적개변,저가능시1,2-DCE신경독성작용적궤제지일.
Objective To explore the effects of 1,2-dichloroethane (1,2-DCE) on the behavior and the brain neurotransmitter levels in mice.Methods Thirty mice were randomly divided into four groups,which were control group and groups of low,meddle and high exposure (225,450 and 900 mg/m3) to 1,2-DCE for 10 days (3.5 h a day) by inhalation.After the last exposure,the open field test was performed immediately.After exposure all mice were killed and the brain tissues were taken up rapidly.The levels of aspartate (Asp),glutamate (Glu) and gamma-aminobutyric acid (GABA) in the brain were detected by high performance liquid chromatography (HPLC ).Results Levels of Asp and Glu in all exposure groups increased with doses.As compared to the control group,levels of Glu in all exposure groups increased significantly (P<0.05 ).Levels of GABA in the low exposure group were significantly lower than those in control group,but those in the high exposure group were significantly higher than those in control group.The results of the open field test showed that effect of low exposure to 1,2-DCE on the behavior was stimulant,but the high exposure to 1,2-DCE inhibited behavior of exploration,excitement and sport.Conclusions Subacute exposure to 1,2-DCE could result in the change of amino acid neurotransmitter content and ratio in the brain,thereby change the behavior of mice appeared,which might be the mechanism of neurotoxicity caused by 1,2-DCE in part.
