国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2009年
11期
646-649
,共4页
姚静婵%应延风%胡野%屠平光
姚靜嬋%應延風%鬍野%屠平光
요정선%응연풍%호야%도평광
支气管哮喘%树突状细胞%CRTH2
支氣管哮喘%樹突狀細胞%CRTH2
지기관효천%수돌상세포%CRTH2
Bronchial asthma%Dendritic cells%Chemokine receptor-homologs molecule expressed on TH2 cells
目的 研究不同病期支气管哮喘(简称哮喘)患儿单核细胞衍生的树突状细胞(dendriticcells,DC)上前列腺素D2受体(DP/CRTH2)结合容量改变,探讨其对Th2极化的影响.方法 随机病例对照研究.分哮喘急性发作期组32例.平均年龄(6.1±3.5)岁.缓解期组30例,平均年龄(5.8±2.6)岁,正常对照组34例,平均年龄(7.2±3.8)岁.用放射配体结合分析法测定外周血DC上DP1/CRTH2结合容量;用酶联免疫吸附试验(ELISA)法测定外周血白介素4(IL-4)、IL-5和γ干扰素(IFN-γ).结果 哮喘发作期和缓解期组DC上总结合和CRTH2受体结合容量比正常对照组显著增加,DP总结合为:(532.42±123.87)fmol/106细胞、(491.78±110.64)fmol/106细胞、(274.61±87.16)fmol/106细胞;CRTH2为:(355.66±99.19)fmol/106细胞、(316.83±81.42)fmol/106细胞、(123.74±49.27)fmol/106细胞;而DP1差异无统计学意义(P<0.01).发作期和缓解期组Th2细胞因子IL-4、IL-5比正常对照组显著增加,IL-4为:(12.76±3.54)ng/L、(11.44±2.65)ng/L、(7.49±1.45)ng/L;IL-5为:(36.28±10.21)ng/L、(32.11±11.56)ng/L、(18.484±5.65)ng/L(P<0.01);发作期和缓解期组Th1细胞因子IFN-γ比正常对照组显著减少,IFN-γ为:(7.47±2.49)ng/L、(7.56±1.41)ng/L、(13.13±1.44)ng/L(P<0.01).结论 哮喘患儿发作期和缓解期外周血DC上CRTH2受体表达和Th2细胞因子IL-4、IL-5分泌增加.
目的 研究不同病期支氣管哮喘(簡稱哮喘)患兒單覈細胞衍生的樹突狀細胞(dendriticcells,DC)上前列腺素D2受體(DP/CRTH2)結閤容量改變,探討其對Th2極化的影響.方法 隨機病例對照研究.分哮喘急性髮作期組32例.平均年齡(6.1±3.5)歲.緩解期組30例,平均年齡(5.8±2.6)歲,正常對照組34例,平均年齡(7.2±3.8)歲.用放射配體結閤分析法測定外週血DC上DP1/CRTH2結閤容量;用酶聯免疫吸附試驗(ELISA)法測定外週血白介素4(IL-4)、IL-5和γ榦擾素(IFN-γ).結果 哮喘髮作期和緩解期組DC上總結閤和CRTH2受體結閤容量比正常對照組顯著增加,DP總結閤為:(532.42±123.87)fmol/106細胞、(491.78±110.64)fmol/106細胞、(274.61±87.16)fmol/106細胞;CRTH2為:(355.66±99.19)fmol/106細胞、(316.83±81.42)fmol/106細胞、(123.74±49.27)fmol/106細胞;而DP1差異無統計學意義(P<0.01).髮作期和緩解期組Th2細胞因子IL-4、IL-5比正常對照組顯著增加,IL-4為:(12.76±3.54)ng/L、(11.44±2.65)ng/L、(7.49±1.45)ng/L;IL-5為:(36.28±10.21)ng/L、(32.11±11.56)ng/L、(18.484±5.65)ng/L(P<0.01);髮作期和緩解期組Th1細胞因子IFN-γ比正常對照組顯著減少,IFN-γ為:(7.47±2.49)ng/L、(7.56±1.41)ng/L、(13.13±1.44)ng/L(P<0.01).結論 哮喘患兒髮作期和緩解期外週血DC上CRTH2受體錶達和Th2細胞因子IL-4、IL-5分泌增加.
목적 연구불동병기지기관효천(간칭효천)환인단핵세포연생적수돌상세포(dendriticcells,DC)상전렬선소D2수체(DP/CRTH2)결합용량개변,탐토기대Th2겁화적영향.방법 수궤병례대조연구.분효천급성발작기조32례.평균년령(6.1±3.5)세.완해기조30례,평균년령(5.8±2.6)세,정상대조조34례,평균년령(7.2±3.8)세.용방사배체결합분석법측정외주혈DC상DP1/CRTH2결합용량;용매련면역흡부시험(ELISA)법측정외주혈백개소4(IL-4)、IL-5화γ간우소(IFN-γ).결과 효천발작기화완해기조DC상총결합화CRTH2수체결합용량비정상대조조현저증가,DP총결합위:(532.42±123.87)fmol/106세포、(491.78±110.64)fmol/106세포、(274.61±87.16)fmol/106세포;CRTH2위:(355.66±99.19)fmol/106세포、(316.83±81.42)fmol/106세포、(123.74±49.27)fmol/106세포;이DP1차이무통계학의의(P<0.01).발작기화완해기조Th2세포인자IL-4、IL-5비정상대조조현저증가,IL-4위:(12.76±3.54)ng/L、(11.44±2.65)ng/L、(7.49±1.45)ng/L;IL-5위:(36.28±10.21)ng/L、(32.11±11.56)ng/L、(18.484±5.65)ng/L(P<0.01);발작기화완해기조Th1세포인자IFN-γ비정상대조조현저감소,IFN-γ위:(7.47±2.49)ng/L、(7.56±1.41)ng/L、(13.13±1.44)ng/L(P<0.01).결론 효천환인발작기화완해기외주혈DC상CRTH2수체표체화Th2세포인자IL-4、IL-5분비증가.
Objective To investigate the change of prostaglandin D2 (DP) receptor on dendritic cells (DC) in exacerbation and remission phase patients with bronchial asthma(asthma) and the ability of receptor to control Th2 polarization. Methods Randomized case control clinical trial was designed. Thirty patients were randomly divided into two groups:exacerbation phase group 32 cases (6.1 ±3.5) years,and remissio nphase group 30 cases (5.8 ± 2.6) years, normal control 34 cases (7. 2± 3.8) years. Radiological binding assay was used to study the DP1/CRTH2 receptors in monocyte-derived DC in peripheral blood. The plasma levels of IL(interleukin) -4,IL-5,and INF-γ were detected by ELISA method. Results The total combining capacity and CRTH2 on DC in peri-pheral blood in exacerbation phase and remission phase group were significantly higher than those in normal controls,total combining capacity: (532.42±123.87) fmol/106cells,(491.781±10. 64) fmol/106 cells,(274.61±87.16) fmol/106 cells. CRTH2:(355.664±99. 19) fmol/106cells,(316. 834±81.42) fmol/106 ce113,(123. 744±49. 27) fmol/106 cells (P <0.01). In three groups,there were no significant differences in DP1. In exacerbation phase and remission phase group, the serum levels of Th2 cytokines IL-4, IL-5 were significantly more increased than in control group, IL-4: (12. 76 4± 3. 54) ng/L,(11. 445±2.65) rig/L, (7.495± 1.45) ng/L. IL-5 : (36.28d±10. 21) ng/L, (32. 11 5±11.56) ng/L, (18. 48 ±5.65) ng/L(P <0. 01). Thl cytokine IFN-γ was significantly decreased in the above three groups, (7. 47 ±2.49) ng/L,(7.565±1.41) ng/L, (13.13 4±1. 44) ng/L(P <0.01). Conclusions There are significantly higher total combining capacity DP and CRTH2 on monocyte-derived DC in exacerbation and remission phase patients with asthma,and the serum levels of Th2 cytokine IL-4,IL-5 are significantly increased.
