CAJ | 학술논문

目的研究HIV/AIDS合并早期隐性梅毒患者外周血T淋巴细胞及其激活亚群的变化,探讨其临床意义.方法用流式细胞仪检测78例HIV/AIDS患者、66例HIWAIDS合并早期隐性梅毒患者和30名健康体检者的T淋巴细胞及其激活亚群( HLA-DR+ CD3 +/CD3+、HLA-DR+CD4 +/CD4+和HLA-DR+ CD8 +/CD8+),同时行梅毒快速血浆反应素试验(RPR)和梅毒螺旋体明胶凝集试验(TPPA)检测.采用SPSS 13.0软件进行统计分析,两样本均数的比较采用t检验(正态分布)或Mann-Whitney U检验(偏态分布).结果 HIV/AIDS合并梅毒患者CD4+T淋巴细胞计数显著高于HIV/AIDS组(t=2.041和2.223,P值均＜0.05),但在CD3+T和CD8 +T淋巴细胞计数方面差异无统计学意义(tcD3 =0.362和0.692,tcD8=0.043和0.617,P值均＞0.05).AIDS合并梅毒组HLA-DR+CD4+/CD4+显著高于HIV合并梅毒组(t=2.647,P＜0.05),但在HLA-DR+ CD3 +/CD3+和HLA-DR+CD8 +/CD8+方面两组比较差异无统计学意义(t=1.112和0.093,P值均＞0.05).结论 HIV/AIDS患者感染隐性梅毒早期细胞免疫功能存在一过性增强的可能.
목적 연구HIV/AIDS합병조기은성매독환자외주혈T림파세포급기격활아군적변화,탐토기림상의의.방법 용류식세포의검측78례HIV/AIDS환자、66례HIWAIDS합병조기은성매독환자화30명건강체검자적T림파세포급기격활아군( HLA-DR+ CD3 +/CD3+、HLA-DR+CD4 +/CD4+화HLA-DR+ CD8 +/CD8+),동시행매독쾌속혈장반응소시험(RPR)화매독라선체명효응집시험(TPPA)검측.채용SPSS 13.0연건진행통계분석,량양본균수적비교채용t검험(정태분포)혹Mann-Whitney U검험(편태분포).결과 HIV/AIDS합병매독환자CD4+T림파세포계수현저고우HIV/AIDS조(t=2.041화2.223,P치균＜0.05),단재CD3+T화CD8 +T림파세포계수방면차이무통계학의의(tcD3 =0.362화0.692,tcD8=0.043화0.617,P치균＞0.05).AIDS합병매독조HLA-DR+CD4+/CD4+현저고우HIV합병매독조(t=2.647,P＜0.05),단재HLA-DR+ CD3 +/CD3+화HLA-DR+CD8 +/CD8+방면량조비교차이무통계학의의(t=1.112화0.093,P치균＞0.05).결론 HIV/AIDS환자감염은성매독조기세포면역공능존재일과성증강적가능.
Objective To investigate the expression of T lymphocytes and activated subsets in patients with HIV/AIDS and early latent syphilis. Methods T lymphocytes and activated subsets ( HLADR+ CD3 +/CD3 + , HLA-DR+ CD4 +/CD4 + and HLA-DR+ CD8 +/CD8 + ) as well as rapid plasma reagin (RPR) and treponema pallidum particle agglutination (TPPA) test were detected by flow cytometry in 78 patients with HIV/AIDS, 66 patients with HIV/AIDS and early latent syphilis, and 30 healthy subjects. SPSS 13.0 was used for statistical analysis, and t (for normal distribution) or Mann-Whitney U (for skew distribution) tests were performed to compare between the groups. Results The absolute counts of CD4+ T cells in patients with HIV/AIDS and early latent syphilis were significantly higher than those in HIV/AIDS patients ( t = 2. 041 and 2. 223, P ＜ 0.05 ), but no difference in the counts of CD3 + T cells and CD8 + T cells was observed (tcD3 =0. 362 and 0.692, tcD8 =0.043 and 0.617, P＞0.05). HLA-DR+ CD4 +/CD4 +level in AIDS plus syphilis group was much higher than that in HIV plus syphilis group ( t = 2. 647, P ＜ 0. 05 ), but no difference was observed in HLA-DR+ CD3 +/CD3 + and HLA-DR+ CDs +/CDs + ( t = 1. 112 and 0. 093, P ＞ 0.05). Conclusions Immune function in patients with HIV/AIDS and early latent syphilis may be enhanced temporarily.