CAJ | 학술논문

目的研究骨髓异常增生综合征(myelodysplastic syndromes,MDS)难治性贫血(refractory anemia,RA)型患者(MDS-RA)和健康成年人骨髓来源的间充质干细胞(bone marrow derived mesenchymal stem cell,BMSC)的免疫学特征,比较它们是否存在免疫功能的异常.方法分离MDS-RA和健康成年人来源的间充质干细胞,分别检测它们对T细胞周期、活化、抑制和增殖的作用情况.结果 MDS-RA的BMSC抑制T细胞增殖和活化能力均减弱,但是抑制活化的T细胞凋亡的作用反而增强.结论 MDS-RA患者骨髓来源的MSC存在明显的免疫调节功能缺陷,MDS-RA患者骨髓微环境可能存在免疫异常,如果使用MDS患者自体的MSC移植治疗可能不是一种很好的选择.对于MDS患者最好是选用异基因的MSC移植.
목적 연구골수이상증생종합정(myelodysplastic syndromes,MDS)난치성빈혈(refractory anemia,RA)형환자(MDS-RA)화건강성년인골수래원적간충질간세포(bone marrow derived mesenchymal stem cell,BMSC)적면역학특정,비교타문시부존재면역공능적이상.방법 분리MDS-RA화건강성년인래원적간충질간세포,분별검측타문대T세포주기、활화、억제화증식적작용정황.결과 MDS-RA적BMSC억제T세포증식화활화능력균감약,단시억제활화적T세포조망적작용반이증강.결론 MDS-RA환자골수래원적MSC존재명현적면역조절공능결함,MDS-RA환자골수미배경가능존재면역이상,여과사용MDS환자자체적MSC이식치료가능불시일충흔호적선택.대우MDS환자최호시선용이기인적MSC이식.
Objective To investigate that whether the immuno-modulatory capacity is intact in myelodysplastic syndrome-refractory anemia (MDS-RA) derived mesenchymal stem cell (MSCs) and what are the differences compare with those from normal donors.Methods Isolating MSC from MDS and normal donors respectively and exam their differences on T lymphocyte activation,proliferation and suppression.Results The results showed that the capacity of suppressing T cell proliferation and activation is weakened.Conclusion We propose that MSCs from pathological environment might be abnormal and should not be used for autologous transplantation.