CAJ | 학술논문

目的对6A8 α-甘露糖苷酶基因作染色体定位，比较6A8 α-甘露糖苷酶mRNA在人多种免疫细胞株的表达。方法用Fish原位杂交作染色体定位，用RT-PCR检测mRNA表达。结果 6A8 α-甘露糖苷酶基因定位于人第13号染色体长臂的31～32区。6A8 α-甘露糖苷酶mRNA在B细胞株SKW 6高表达，在B细胞株3D5、BJAB、CESS、Daudi、Nalm 6中度表达，在B细胞株Navalm，T细胞株GM、Jurkat，组织细胞瘤细胞株U937弱表达，在T细胞株Peer，慢性髓性白血病细胞株K562极弱表达。结论 6A8 α-甘露糖苷酶基因定位于人第13号染色体长臂的31～32区，其mRNA表达水平在各种人免疫细胞株不同。
목적대6A8 α-감로당감매기인작염색체정위，비교6A8 α-감로당감매mRNA재인다충면역세포주적표체。방법용Fish원위잡교작염색체정위，용RT-PCR검측mRNA표체。결과 6A8 α-감로당감매기인정위우인제13호염색체장비적31～32구。6A8 α-감로당감매mRNA재B세포주SKW 6고표체，재B세포주3D5、BJAB、CESS、Daudi、Nalm 6중도표체，재B세포주Navalm，T세포주GM、Jurkat，조직세포류세포주U937약표체，재T세포주Peer，만성수성백혈병세포주K562겁약표체。결론 6A8 α-감로당감매기인정위우인제13호염색체장비적31～32구，기mRNA표체수평재각충인면역세포주불동。
Objective　To localize 6A8 α-mannosidase gene in chromosome and to compare the 6A8 α-mannosidase mRNA expression in various human immune cell lines. Methods　Fish and RT-PCR. Results　6A8 α-mannosidase gene is localized in human chromosome 13q31-32. 6A8 α-mannosidase mRNA shows a high expression in B cell line SKW 6, a median expression in B cell line 3D5, BJAB, CESS, Daudi, Nalm 6, a weak expression in B cell line Navalm, T cell line GM, Jurkat and histocyte line U937, and a very weak or no expression in T cell line Peer and a chronic myelogenous leukemia cell line K562. Conclusion　6A8 α-mannosidase gene is localized in human chromosome 13q31-32 and the expression of 6A8 α-mannosidase in mRNA level is variant in different human immune cell lines.