中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2011年
7期
483-486
,共4页
紫外线疗法%皮肤色素沉着%辐射损伤%实验性%一氧化氮合酶
紫外線療法%皮膚色素沉著%輻射損傷%實驗性%一氧化氮閤酶
자외선요법%피부색소침착%복사손상%실험성%일양화담합매
Ultraviolet therapy%Skin pigmentation%Radiation injuries,experimental%Nitric-oxide synthase
目的 观察中波高能紫外线对皮肤色素沉着的诱导作用及其色素增加的作用机制.方法 以正常棕黄色豚鼠皮肤为实验模型,在其背部脱毛处理后,取4个相邻的区域依次作为对照组和低、中、高剂量中波高能紫外线照射组,分别采用肉眼评估、黑素细胞染色(Imokawa法)以及黑素颗粒染色(Masson-Fontana法)方法,研究中波高能紫外线所致色素沉着作用;应用免疫组化染色法对不同照射剂量组豚鼠皮肤中的一氧化氮合酶(NOS)进行检测;HE染色观察表皮组织学改变.结果 各照射组均出现不同程度的色素沉着,除中、高剂量组色素沉着评分结果差异无统计学意义外,其余各组的色素沉着评分平均黑素数量以及多巴阳性黑素细胞数之间的差异均具有统计学意义(P<0.05),且中剂量组结果高于高剂量组.诱导型NOS的表达呈照射剂量依赖式递增,对照组和低、中、高剂量组诱导型NOS含量评分中位数分别为0.50、1.25、1.75、2.00,组问差异具有统计学意义(P<0.05).结论 中波高能紫外线可诱导豚鼠皮肤色素增加,且这种效应有一定的剂量依赖性,但并非剂量越高越好.短期内接受中波高能紫外线照射是安全有效的.
目的 觀察中波高能紫外線對皮膚色素沉著的誘導作用及其色素增加的作用機製.方法 以正常棕黃色豚鼠皮膚為實驗模型,在其揹部脫毛處理後,取4箇相鄰的區域依次作為對照組和低、中、高劑量中波高能紫外線照射組,分彆採用肉眼評估、黑素細胞染色(Imokawa法)以及黑素顆粒染色(Masson-Fontana法)方法,研究中波高能紫外線所緻色素沉著作用;應用免疫組化染色法對不同照射劑量組豚鼠皮膚中的一氧化氮閤酶(NOS)進行檢測;HE染色觀察錶皮組織學改變.結果 各照射組均齣現不同程度的色素沉著,除中、高劑量組色素沉著評分結果差異無統計學意義外,其餘各組的色素沉著評分平均黑素數量以及多巴暘性黑素細胞數之間的差異均具有統計學意義(P<0.05),且中劑量組結果高于高劑量組.誘導型NOS的錶達呈照射劑量依賴式遞增,對照組和低、中、高劑量組誘導型NOS含量評分中位數分彆為0.50、1.25、1.75、2.00,組問差異具有統計學意義(P<0.05).結論 中波高能紫外線可誘導豚鼠皮膚色素增加,且這種效應有一定的劑量依賴性,但併非劑量越高越好.短期內接受中波高能紫外線照射是安全有效的.
목적 관찰중파고능자외선대피부색소침착적유도작용급기색소증가적작용궤제.방법 이정상종황색돈서피부위실험모형,재기배부탈모처리후,취4개상린적구역의차작위대조조화저、중、고제량중파고능자외선조사조,분별채용육안평고、흑소세포염색(Imokawa법)이급흑소과립염색(Masson-Fontana법)방법,연구중파고능자외선소치색소침착작용;응용면역조화염색법대불동조사제량조돈서피부중적일양화담합매(NOS)진행검측;HE염색관찰표피조직학개변.결과 각조사조균출현불동정도적색소침착,제중、고제량조색소침착평분결과차이무통계학의의외,기여각조적색소침착평분평균흑소수량이급다파양성흑소세포수지간적차이균구유통계학의의(P<0.05),차중제량조결과고우고제량조.유도형NOS적표체정조사제량의뢰식체증,대조조화저、중、고제량조유도형NOS함량평분중위수분별위0.50、1.25、1.75、2.00,조문차이구유통계학의의(P<0.05).결론 중파고능자외선가유도돈서피부색소증가,차저충효응유일정적제량의뢰성,단병비제량월고월호.단기내접수중파고능자외선조사시안전유효적.
Objective To study the inductive effect of targeted UVB phototherapy on skin hyperpigmentation and its mechanism.Methods Ten brownish guinea pigs were used to develop experimental models.After depilation,four adjacent areas were selected on the back of each guinea pigs and served as the control,low-dose,moderate-dose and high-dose group to receive targeted UVB irradiation with a cumulative dose of O,2500,3500,4500 mJ/cm2,respectively.After 6-week irradiation,the guinea pigs were sacrificed and skin sampies were obtained.The hyperpigmentation induced by UVB irradiation was estimated by naked eyes,staining for melanocytes(Imokawa method)and melanin granules(Masson-Fontana staining),respectively.Immunohistochemistry was carried out to determine the level of nitric oxide synthase and HE staining to observe epidermal histological changes.Results A statistical difference was observed in the pigmentation score,quantity of melanin granules and dopa-positive melanocyte number among the four groups (P<0.05),and the moderatedose group was higher than the high-dose group in terms of these parameters.The expression of inducible nitric oxide synthase increased in a radiation dose-dependent manner,and the median value for inducible nitric oxide synthase expression level was 0.50,1.25,1.75,2.00 in the control,low-dose,moderate-dose and highdose group,respectively(P<0.05).Conclusions Targeted UVB phototherapy can induce hyperpigmentation of the skin in brownish guinea pigs in a dose-dependent manner,but higher dose may not work better.To irradiate with an initial dose close to or slightly higher than the minimum erythema dose may result in a satisfactory effect with reduced cumulative dose and potential risk for cancer.
