CAJ | 학술논문

目的观察1-溴丙烷(1-bromopropane,1-BP)对大鼠学习记忆功能及对胆碱能神经系统的影响.方法雄性Wistar大鼠40只,随机分为4组:1-BP低剂量组(200mg/kg1-BP)、中剂量组(400mg/kg1-BP)、高剂量组(800mg/kg 1-BP)和对照组,每组10只.各组动物分别经灌胃给予相应受试物7 d后,采用Morris水迷宫定位航行试验和空间探索试验检测大鼠学习和记忆功能.水迷宫试验结束后次日断头处死大鼠,迅速剥离大鼠大脑和海马,冰浴中制备匀浆,取上清液,测定乙酰胆碱酯酶(AChE)、乙酰胆碱转移酶(ChAT)的活力.结果定位航行试验中,1-BP染毒后,与对照组相比,中、高剂基组的逃避潜伏期、游泳路程明显延长,搜索效能降低,差异均有统计学意义(P＜0.05,P＜0.01).空间探索试验中,低、中、高剂量组穿越平台次数(分别为4.30±2.6、3.78±2.0、2.50±2.1)呈降低趋势,与对照组(7.20±2.8)比较,差异均有统计学意义(P＜0.05,P＜0.01),高剂量组的平台周边时间/总时间比值(0.55±0.14)明显低于对照组(0.76±0.15)和低剂量组(0.69±0.18),差异有统计学意义(P＜0.01).中、高剂量组大鼠大脑皮层AChE活力[分别为(1.246±0.423)、(1.397±0.503)U/mgpro]明显升高,与对照组[(0.918±0.276)U/mg pro]的差异有统计学意义(P＜0.05,P＜0.01),高剂量组海马组织中AChE活力[(0.583±0.118)U/mgpro]也明显高于对照组[(0.491±0.075)U/mgpro],差异有统计学意义(P＜0.05).1-BP染毒后,大脑皮层ChAT活力有降低趋势,但与对照组相比,差异无统计学意义(P＞0.05).结论 1-BP暴露能明显损伤大鼠学习记忆能力,可能与AChE活力升高相关.
목적 관찰1-추병완(1-bromopropane,1-BP)대대서학습기억공능급대담감능신경계통적영향.방법 웅성Wistar대서40지,수궤분위4조:1-BP저제량조(200mg/kg1-BP)、중제량조(400mg/kg1-BP)、고제량조(800mg/kg 1-BP)화대조조,매조10지.각조동물분별경관위급여상응수시물7 d후,채용Morris수미궁정위항행시험화공간탐색시험검측대서학습화기억공능.수미궁시험결속후차일단두처사대서,신속박리대서대뇌화해마,빙욕중제비균장,취상청액,측정을선담감지매(AChE)、을선담감전이매(ChAT)적활력.결과 정위항행시험중,1-BP염독후,여대조조상비,중、고제기조적도피잠복기、유영로정명현연장,수색효능강저,차이균유통계학의의(P＜0.05,P＜0.01).공간탐색시험중,저、중、고제량조천월평태차수(분별위4.30±2.6、3.78±2.0、2.50±2.1)정강저추세,여대조조(7.20±2.8)비교,차이균유통계학의의(P＜0.05,P＜0.01),고제량조적평태주변시간/총시간비치(0.55±0.14)명현저우대조조(0.76±0.15)화저제량조(0.69±0.18),차이유통계학의의(P＜0.01).중、고제량조대서대뇌피층AChE활력[분별위(1.246±0.423)、(1.397±0.503)U/mgpro]명현승고,여대조조[(0.918±0.276)U/mg pro]적차이유통계학의의(P＜0.05,P＜0.01),고제량조해마조직중AChE활력[(0.583±0.118)U/mgpro]야명현고우대조조[(0.491±0.075)U/mgpro],차이유통계학의의(P＜0.05).1-BP염독후,대뇌피층ChAT활력유강저추세,단여대조조상비,차이무통계학의의(P＞0.05).결론 1-BP폭로능명현손상대서학습기억능력,가능여AChE활력승고상관.
Objective To study the effects of 1-bromopropane (1-BP) on the functions of learning-memory and the central cholinergic system in rats.Methods Forty male Wistar rats were randomly divided into four groups:low 1-BP group (200 mg/kg), middle 1-BP group (400 mg/kg), high 1-BP group ( 800 mg/kg) and control group, and the exposure time was 7 days.The Morris water maze (MWM) test was applied to evaluate the learning-memory function in rats.After the MWM test, the rats were sacrificed, the cerebral cortex and hippocampus were quickly dissected and homogenized in ice bath.The activity of acetylcholine esterase (AChE) and choline acety]transferase (ChAT) in supernatant of homogenate were detected.Results The latency and swim path-length of rats in middle and high 1-BP groups prolonged significantly in place navigation test and the efficiency of searching strategy obviously decreased, as compared with control group (P＜0.05 or P＜0.01 ).In spatial probe test, the number of crossing platform in three 1-BP groups decreased significantly, as compared with control group (P＜0.05 or P＜0.01 ).The cortical AChE activity of rats in middle and high 1-BP groups was significantly higher than that of control and low 1-BP group (P＜0.05 or P＜0.01 ).The AChE activity in rat hippocampus of high 1-BP group obviously increased, as compared with control group as compared with control group (P＜0.05).There was no significant difference of cortical ChAT activity between three 1-BP groups and control group ( P＞0.05 ).In the hippocampus, there was no difference of ChAT activity among the groups (P＞0.05).Conclusion 1-BP exposure could significantly influence the learning-memory function in rats due to the increase of AChE activity.