CAJ | 학술논문

目的监测核苷类似物干预下HBV前S1抗原(PreS1)、前S2抗原(PreS2)的动态变化,探讨PreS1、PreS2与HBV DNA复制水平的相关性.方法选取拉米夫定治疗有效应答后出现病毒学反跳的慢性乙型肝炎患者12例,以及持续5年阿德福韦酯抗病毒治疗的慢性乙型肝炎患者20例,动态监测PreS1、PreS2及其他HBV标志物.根据不同资料分别选择t检验、X2检验、线性相关与回归分析或方差分析.结果 12例拉米夫定治疗的患者,在HBV DNA发生剧烈变化的4个时间点,均未观察到PreS1、PreS2滴度随HBV DNA复制水平的一致变化;20例阿德福韦酯治疗的患者中,仅1例患者PreS1、PreS2随HBV DNA和HBeAg一起阴转,其余均无显著变化;采用不等距重复测量设计方差分析绘制变化趋势轮廓图,未观察到PreS1、PreS2滴度随HBV DNA水平一致的变化趋势.结论在核苷类似物干预下PreS1、PreS2滴度不能反映HBV DNA复制水平以及HBeAg的血清学转换.
목적 감측핵감유사물간예하HBV전S1항원(PreS1)、전S2항원(PreS2)적동태변화,탐토PreS1、PreS2여HBV DNA복제수평적상관성.방법 선취랍미부정치료유효응답후출현병독학반도적만성을형간염환자12례,이급지속5년아덕복위지항병독치료적만성을형간염환자20례,동태감측PreS1、PreS2급기타HBV표지물.근거불동자료분별선택t검험、X2검험、선성상관여회귀분석혹방차분석.결과 12례랍미부정치료적환자,재HBV DNA발생극렬변화적4개시간점,균미관찰도PreS1、PreS2적도수HBV DNA복제수평적일치변화;20례아덕복위지치료적환자중,부1례환자PreS1、PreS2수HBV DNA화HBeAg일기음전,기여균무현저변화;채용불등거중복측량설계방차분석회제변화추세륜곽도,미관찰도PreS1、PreS2적도수HBV DNA수평일치적변화추세.결론 재핵감유사물간예하PreS1、PreS2적도불능반영HBV DNA복제수평이급HBeAg적혈청학전환.
Objective To investigate the dynamic correlation between pre-S1 antigen.pre-S2 antigen and HBV DNA in the serum of chronic hepatitis B(CHB)patients undergoing nucleoside analogue therapy.Methods 12 CHB patients with transient virological response after lamivudine treatment.and 20 patients treated with adefovir for 5 years were recruited in this study.Serum samples were collected at four time points when HBV DNA fluctuated sharply dunng lamivudine treatment,and at 0,8,12,28,52,104,156,208,260 weeks following adefovir treatment.HBV DNA was quantified by real-time PCR,pre-S1 and pre-S2 antigens were detected by ELISA.Results The titers of pre-S1 and pre-S2 antigens were not correlated with the HBV DNA level in the serum of lamivudine treated patients.Only in one case of the adfovir treated patients.the decrease of pre-S1 and pre-S2 antigens was in parallel with the decrease of HBV DNA.Linear regression analysis indicated that neither pre-S1 antigen nor pre-S2 antigen was correlated with HBV DNA in the serum of lamivudine or adfovir treated patients(P＞0.05).Conclusion Our results indicate that the titers of pre-S1 and pre-S2 antigens are not correlated with the serum HBV DNA in CHB patients undergoing nucleoside analogue therapy.Neither pre-S1 nor pre-S2 is a good predictor for the outcome of nucleoside analogue treatment.