CAJ | 학술논문

目的建立超大剂量γ线离体照射后人血淋巴细胞早熟凝集染色体环(PCC-R)的剂量.效应曲线.方法外周静脉血样采自3名健康男性,经60Co γ线(剂量率2.35 Gy/min)照射0、1、3、5、8、10、13、16、20、26和30 Gy.于37℃恒温箱培养48 h,收获前1 h加入冈田酸以诱导产生早熟凝集染色体(PCC),计数PCC细胞中的PCC-R频率,拟合剂量-效应曲线.结果 PCC指数随照射剂量增加而逐渐减少;每细胞PCC-R频率随照射剂量增加而增加直到20 Gy,>20 Gy后趋于饱和.对所获数据进行回归分析,所拟合的曲线符合线性模型(上限剂量到20 Cy):y=-0.020+0.052D(y为每细胞PCC-R频率,D为剂量,Gy).结论本研究用药物诱导PCC-R法所建立的剂量-效应曲线可估计的上限剂量达20 Gy,它的可靠性和实用性还有待于在今后的辐射事故中实际应用加以验证.
목적 건립초대제량γ선리체조사후인혈림파세포조숙응집염색체배(PCC-R)적제량.효응곡선.방법 외주정맥혈양채자3명건강남성,경60Co γ선(제량솔2.35 Gy/min)조사0、1、3、5、8、10、13、16、20、26화30 Gy.우37℃항온상배양48 h,수획전1 h가입강전산이유도산생조숙응집염색체(PCC),계수PCC세포중적PCC-R빈솔,의합제량-효응곡선.결과 PCC지수수조사제량증가이축점감소;매세포PCC-R빈솔수조사제량증가이증가직도20 Gy,>20 Gy후추우포화.대소획수거진행회귀분석,소의합적곡선부합선성모형(상한제량도20 Cy):y=-0.020+0.052D(y위매세포PCC-R빈솔,D위제량,Gy).결론 본연구용약물유도PCC-R법소건립적제량-효응곡선가고계적상한제량체20 Gy,타적가고성화실용성환유대우재금후적복사사고중실제응용가이험증.
Objective To establish a dose-effect curve of premature condensation chromosome ring (PCC-R)in lymphocytes of human peripheral blood after exposed to high doses of Y-rays. Methods Peripheral blood samples was drawn from three healthy individuals, and exposed to 60Co γ-rays with doses between 0 and 30 Gy. The frequencies of PCC-R in premature condensation chromosome (PCC) cells obtained by Okadaic acid (OA) induction were calculated, and a dose-effect curve was fitted. Results PCC index tapered with dose. Frequencies of PCC-R per cell increased until 20 Gy, and then saturation was observed. The results were fitted to a lineal model up to 20 Gy: y = - 0.020 + 0.052D ,where y was the frequencies of PCC-R per cell, D was the radiation dose (Gy). Conclusins The highest dose could be estimated is 20 Gy by the dose-effect curve established with PCC-R method. Its utility and validity will be verified in the future application of radiation accident.