中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2010年
7期
13-16
,共4页
陶松桔%张亚萍%惠辉%伍蔚凌
陶鬆桔%張亞萍%惠輝%伍蔚凌
도송길%장아평%혜휘%오위릉
糖尿病,2型%胰岛素输注系统%氧化性应激%内皮祖细胞
糖尿病,2型%胰島素輸註繫統%氧化性應激%內皮祖細胞
당뇨병,2형%이도소수주계통%양화성응격%내피조세포
Diabetes mellitus,type 2%Insulin infusion systems%Oxidative stress%Endothelial progenitor cells
目的 观察短期胰岛素泵(CSⅡ)强化治疗对初诊2型糖尿病(T2DM)患者外周血循环内皮祖细胞(EPCs)的保护作用及其抗氧化机制.方法 选取初诊T2DM患者40例(T2DM组),健康体检者30例(对照组),采用密度梯度离心法收集外周血单个核细胞,诱导分化培养7 d后,激光共聚焦显微镜鉴定FITC-UEA和DIL-acLDL双染色阳性细胞为正在分化的EPCs,并在倒置显微镜下计数.采用半自动生化仪测定血清活性氧活力(ROS)、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD).结果 与对照组比较,T2DM组外周血EPCs减少[(46±3)个比(23±4)个](P<0.01),ROS、MDA增高[分别为(1125±687)U/ml比(2088±765)U/ml、(4.58±3.03)μmol/L比(8.32±4.15)μmol/L](P<0.01),GSH、SOD减低[分别为(263 ±35)mg/L比(206±41)mg/L、(103±7)U/ml比(80±15)U/ml](P<0.05).EPCs与ROS、MDA呈负相关(r=-0.7352,P<0.01;r=-0.8454,P<0.01),与GSH、SOD呈正相关(r=0.5435,P<0.05;r=0.6437,P<0.05).经短期CSⅡ强化治疗后T2DM患者EPCs明显增加(P<0.05),ROS、MDA明显降低(P<0.05),GSH、SOD明显增加(P<0.05).结论 短期CSⅡ强化治疗可明显减轻T2DM患者体内氧化应激,促进EPCs数量的恢复,进而防止血管病变.
目的 觀察短期胰島素泵(CSⅡ)彊化治療對初診2型糖尿病(T2DM)患者外週血循環內皮祖細胞(EPCs)的保護作用及其抗氧化機製.方法 選取初診T2DM患者40例(T2DM組),健康體檢者30例(對照組),採用密度梯度離心法收集外週血單箇覈細胞,誘導分化培養7 d後,激光共聚焦顯微鏡鑒定FITC-UEA和DIL-acLDL雙染色暘性細胞為正在分化的EPCs,併在倒置顯微鏡下計數.採用半自動生化儀測定血清活性氧活力(ROS)、丙二醛(MDA)、穀胱甘肽(GSH)、超氧化物歧化酶(SOD).結果 與對照組比較,T2DM組外週血EPCs減少[(46±3)箇比(23±4)箇](P<0.01),ROS、MDA增高[分彆為(1125±687)U/ml比(2088±765)U/ml、(4.58±3.03)μmol/L比(8.32±4.15)μmol/L](P<0.01),GSH、SOD減低[分彆為(263 ±35)mg/L比(206±41)mg/L、(103±7)U/ml比(80±15)U/ml](P<0.05).EPCs與ROS、MDA呈負相關(r=-0.7352,P<0.01;r=-0.8454,P<0.01),與GSH、SOD呈正相關(r=0.5435,P<0.05;r=0.6437,P<0.05).經短期CSⅡ彊化治療後T2DM患者EPCs明顯增加(P<0.05),ROS、MDA明顯降低(P<0.05),GSH、SOD明顯增加(P<0.05).結論 短期CSⅡ彊化治療可明顯減輕T2DM患者體內氧化應激,促進EPCs數量的恢複,進而防止血管病變.
목적 관찰단기이도소빙(CSⅡ)강화치료대초진2형당뇨병(T2DM)환자외주혈순배내피조세포(EPCs)적보호작용급기항양화궤제.방법 선취초진T2DM환자40례(T2DM조),건강체검자30례(대조조),채용밀도제도리심법수집외주혈단개핵세포,유도분화배양7 d후,격광공취초현미경감정FITC-UEA화DIL-acLDL쌍염색양성세포위정재분화적EPCs,병재도치현미경하계수.채용반자동생화의측정혈청활성양활력(ROS)、병이철(MDA)、곡광감태(GSH)、초양화물기화매(SOD).결과 여대조조비교,T2DM조외주혈EPCs감소[(46±3)개비(23±4)개](P<0.01),ROS、MDA증고[분별위(1125±687)U/ml비(2088±765)U/ml、(4.58±3.03)μmol/L비(8.32±4.15)μmol/L](P<0.01),GSH、SOD감저[분별위(263 ±35)mg/L비(206±41)mg/L、(103±7)U/ml비(80±15)U/ml](P<0.05).EPCs여ROS、MDA정부상관(r=-0.7352,P<0.01;r=-0.8454,P<0.01),여GSH、SOD정정상관(r=0.5435,P<0.05;r=0.6437,P<0.05).경단기CSⅡ강화치료후T2DM환자EPCs명현증가(P<0.05),ROS、MDA명현강저(P<0.05),GSH、SOD명현증가(P<0.05).결론 단기CSⅡ강화치료가명현감경T2DM환자체내양화응격,촉진EPCs수량적회복,진이방지혈관병변.
Objective To observe the protective effects of short-term continuous subcutaneous insulin infusion (CSⅡ) therapy on the peripheral blood circulating endothelial progenitor cells (EPCs) in patients with newly diagnosed type 2 diabetes mellitus (T2DM) and its antioxidation mechanism. Methods Forty T2DM patients (T2DM group) and 30 controls (control group) were involved in this study. Density gradient centrifugation was used to collect peripheral blood mononuelear cells. After 7 days' inducement and differentiation, FITC-UEA and DIL-acLDL double staining cells were identified to be differentiated EPCs by laser scanning conform microscope, and they were counted under inverted microscope. Serum levels of reactive oxygen species (ROS), malondialde-hyde (MDA), glutathione (GSH), superoxide dismutase (SOD) were examined. Results In T2DM group, the EPCs in peripheral blood were significantly reduced (46 ± 3 vs 23 ± 4, P< 0.01), BOS and MDA increased[(1125 ± 687) U/ml vs(2088 ± 765) U/ml, (4.58 ±3.03) μ mol/L vs (8.32 ± 4.15) μ mol/L, P < 0.01] , and GSH and SOD decreased [(263 ± 35) mg/L vs (206 ± 41) mg/L, (103 ± 7) U/ml vs (80 ± 15) U/ml, P < 0.05] . EPCs were negatively correlated with BOS and MDA (r = -0.7352,P < 0.01 ;r = -0.8454, P < 0.01) and was positively correlated with GSH and SOD (r =0.5435,P <0.05;r =0.6437,P <0.05). After short-term CSⅡ therapy, EPCs, GSH, and SOD in patients with T2DM significantly increased (P < 0.05),but BOS and MDA significantly decreased (P <0.05). Conclusion Short-term CSⅡ therapy can significantly reduce oxidative stress in patients with T2DM, promote the restoration of EPCs, and then prevent vascular disease.
