国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2012年
3期
165-168,后插7
,共5页
宋丽萍%朱敦皖%刘兰霞%董霞%王海%柏金根%冷希岗
宋麗萍%硃敦皖%劉蘭霞%董霞%王海%柏金根%冷希崗
송려평%주돈환%류란하%동하%왕해%백금근%랭희강
亚甲基磷酸化壳聚糖%非病毒载体%基因转染
亞甲基燐痠化殼聚糖%非病毒載體%基因轉染
아갑기린산화각취당%비병독재체%기인전염
Methylene phosphonic chitosan%Non-viral vector%Gene transfection
目的 考察N-亚甲基磷酸化壳聚糖( NMPCS)基因纳米粒子的体外细胞毒性及基因转染效率.方法 采用均相反应法制备了NMPCS,用复凝聚法制备了NMPCS/DNA纳米粒子；通过MTT实验考察了NMPCS及其与DNA复合物对HeLa细胞的细胞毒性,以荧光索酶质粒为报告基因考察了NMPCS及NMPCS-CaZ+载体介导的体外基因转染效率.结果 NMPCS及其与DNA的复合物在体外表现出很小的细胞毒性,远远低于同等浓度时聚乙烯亚胺(PEI)的毒性.通过对壳聚糖进行亚甲基磷酸化修饰后,可大幅提高载体的基因转染效率.结论 N-亚甲基磷酸化壳聚糖有望成为一种新型、安全、高效的非病毒基因载体.
目的 攷察N-亞甲基燐痠化殼聚糖( NMPCS)基因納米粒子的體外細胞毒性及基因轉染效率.方法 採用均相反應法製備瞭NMPCS,用複凝聚法製備瞭NMPCS/DNA納米粒子；通過MTT實驗攷察瞭NMPCS及其與DNA複閤物對HeLa細胞的細胞毒性,以熒光索酶質粒為報告基因攷察瞭NMPCS及NMPCS-CaZ+載體介導的體外基因轉染效率.結果 NMPCS及其與DNA的複閤物在體外錶現齣很小的細胞毒性,遠遠低于同等濃度時聚乙烯亞胺(PEI)的毒性.通過對殼聚糖進行亞甲基燐痠化脩飾後,可大幅提高載體的基因轉染效率.結論 N-亞甲基燐痠化殼聚糖有望成為一種新型、安全、高效的非病毒基因載體.
목적 고찰N-아갑기린산화각취당( NMPCS)기인납미입자적체외세포독성급기인전염효솔.방법 채용균상반응법제비료NMPCS,용복응취법제비료NMPCS/DNA납미입자；통과MTT실험고찰료NMPCS급기여DNA복합물대HeLa세포적세포독성,이형광색매질립위보고기인고찰료NMPCS급NMPCS-CaZ+재체개도적체외기인전염효솔.결과 NMPCS급기여DNA적복합물재체외표현출흔소적세포독성,원원저우동등농도시취을희아알(PEI)적독성.통과대각취당진행아갑기린산화수식후,가대폭제고재체적기인전염효솔.결론 N-아갑기린산화각취당유망성위일충신형、안전、고효적비병독기인재체.
Objective To investigate the cytotoxicity and gene transfection mediated by NMPCS/DNA nanoparticles.Methods N-methylene phosphonic chitosan (NMPCS) was synthesized using one-step reaction under homogeneous conditions.The NMPCS/DNA nanoparticles were prepared using complex coacervation method.The cytotoxicity of NMPCS alone and its complexes with plasmid DNA were determined by MTT assay on HeLa cells.The gene transfection mediated by NMPCS/DNA nanoparticles were investigated using pGL3control vector as reporter gene.Results The MTT results suggested that the NMPCS and NMPCS/DNA complexes showed significantly lower cytotoxicity than PEI and PEI/DNA complexes,respectively.The gene transfection mediated by NMPCS/DNA nanoparticles were greatly improved compared with unmodified chitosan.Conclusion NMPCS would demonstrate great potential as a novel,safe,efficient non-viral vector for gene delivery.
