CAJ | 학술논문

目的探讨原发性皮肤边缘区B细胞淋巴瘤(PCMZL)中BCL10蛋白的表达和相关染色体的异常.方法收集17例PCMZL,用免疫组化检测BCL10的表达,用荧光原位杂交(FISH)的方法分别检测API2-MALT1、BCL10、MALT1和IgH基因的异常.结果在17例PCMZL中,BCL10的阳性率为94.1%(16/17),其中细胞质阳性率为64.7%(11/17),细胞核阳性率为29.4%(5/17).在FISH检测中,所有病例都不存在t(11;18),t(1;14)和t(14;18)染色体异常.与其他部位的MALT淋巴瘤相比较,在PCMZL中染色体易位的发生率不常见,可能这些遗传学异常不是PCMZL发生中的重要因素,而有其他目前未知的因素参与肿瘤的发生.结论 BCL10的核表达与是否出现上述的染色体异常无关,是否代表更具侵袭性的一种预后标记,尚需长期随访观察.
목적 탐토원발성피부변연구B세포림파류(PCMZL)중BCL10단백적표체화상관염색체적이상.방법 수집17례PCMZL,용면역조화검측BCL10적표체,용형광원위잡교(FISH)적방법분별검측API2-MALT1、BCL10、MALT1화IgH기인적이상.결과 재17례PCMZL중,BCL10적양성솔위94.1%(16/17),기중세포질양성솔위64.7%(11/17),세포핵양성솔위29.4%(5/17).재FISH검측중,소유병례도불존재t(11;18),t(1;14)화t(14;18)염색체이상.여기타부위적MALT림파류상비교,재PCMZL중염색체역위적발생솔불상견,가능저사유전학이상불시PCMZL발생중적중요인소,이유기타목전미지적인소삼여종류적발생.결론 BCL10적핵표체여시부출현상술적염색체이상무관,시부대표경구침습성적일충예후표기,상수장기수방관찰.
Objective To study the expression of BCL10 and associated chromosomal aberration in primary cutaneous marginal zone B-cell lymphoma (PCMZL). Methods Tissue specimens were collected from 17 patients with PCMZL. Immunohistochemistry was used to detect the expression of BCL10. Fluorescence in situ hybridization (FISH) was performed to examine the presence of API2-MALT1 fusion gene and chromosomal aberration in BCL10, MALT1 as well as IgH genes in these cases. Results Of these patients,94.1% (16/17) expressed BCL10 protein. The cytoplasmic expression of BCL10 was observed in 64.7% (11/17) of the patients, and nuclear expression in 29.4% (5/17). As shown by FISH test, neither API2-MALT1 fusion gene nor chromosomal aberration in BCL10, MALT1 or IgH genes was present in these patients. Conclusions Compared with MALT lymphomas originating from tissues other than skin, PCMZL is uncommonly associated with chromosomal abnormalities; it is possible that there are unknown factors contributing to its tumorigenesis. Nuclear BCL10 is unrelated to the presence of chromosomal aberration in BCL10, MALT1 or IgH genes. Further follow-up is required to clarify the association between nucle ar BCL10 and poor prognosis of PCMZL.