CAJ | 학술논문

目的观察鞘内注射硫酸镁( MgSO4)对骨癌痛小鼠痛行为的影响.方法雄性C3 H/HeJ小鼠56只,8 ～10周龄,体质量18～22 g,随机分为7组(n=8):假手术Sham组(S组)、癌痛模型对照Control组(C组)和MgSO4及吗啡Treat组(T1 -T5组).C组和T组(T1-T5组)小鼠右侧股骨远端骨髓腔接种含有2×105个NCTC 2472纤维肉瘤细胞的20μl α-MEM,建立骨癌痛模型；S组小鼠骨髓腔注入不含NCTC2472细胞的20μl α-MEM.在接种肿瘤细胞后第14天S组和C组鞘内注射5μl人工脑脊液,T1 -T5组分别鞘内注射溶于人工脑脊液的MgSO4 14.4 μg/5μl、43.2 μg/5μl、86.4 μg/5μl、吗啡0.36 μg/5μl、MgSO414.4μg-吗啡0.36 μg/5 μl.各组于给药前0.5h及给药后的0.5h、2h、4h、8h检测小鼠的自发抬足次数、机械性缩足反射阈值(PWMT)和热缩足反射潜伏期(PWTL).结果鞘内给予MgSQ 14.4 μg、吗啡0.36 μg对骨癌痛小鼠痛行为无影响,鞘内给予MgSO4 43.2 μg、86.4 μg能剂量依赖性减轻小鼠痛行为反应,同时MgSO4 14.4μg-吗啡阈下剂量0.36μg组合也减轻小鼠痛行为反应,给药后0.5h各组小鼠自发抬足次数[(10.08±1.66)次、(7.35±1.36)次、(10.54±1.32)次]比C组[(13.05±2.06)次]降低,PWMT[ (0.81±0.22)g、(1.33±0.19)g、(0.93±0.26)g]、PWTL[ (10.57±1.53)s、(13.12±1.71)s、(11.46±1.83)s]比C组[(0.42±0.23)g、(8.87±1.27)s]升高,差异有统计学意义(P＜0.05).作用2h达到高峰,维持到4h左右,8h后基本消失.结论鞘内注射MgSO4能够剂量依赖性改善骨癌痛小鼠的痛行为；同时MgSO4可增强阈下剂量吗啡的镇痛作用.
목적 관찰초내주사류산미( MgSO4)대골암통소서통행위적영향.방법 웅성C3 H/HeJ소서56지,8 ～10주령,체질량18～22 g,수궤분위7조(n=8):가수술Sham조(S조)、암통모형대조Control조(C조)화MgSO4급마배Treat조(T1 -T5조).C조화T조(T1-T5조)소서우측고골원단골수강접충함유2×105개NCTC 2472섬유육류세포적20μl α-MEM,건립골암통모형；S조소서골수강주입불함NCTC2472세포적20μl α-MEM.재접충종류세포후제14천S조화C조초내주사5μl인공뇌척액,T1 -T5조분별초내주사용우인공뇌척액적MgSO4 14.4 μg/5μl、43.2 μg/5μl、86.4 μg/5μl、마배0.36 μg/5μl、MgSO414.4μg-마배0.36 μg/5 μl.각조우급약전0.5h급급약후적0.5h、2h、4h、8h검측소서적자발태족차수、궤계성축족반사역치(PWMT)화열축족반사잠복기(PWTL).결과 초내급여MgSQ 14.4 μg、마배0.36 μg대골암통소서통행위무영향,초내급여MgSO4 43.2 μg、86.4 μg능제량의뢰성감경소서통행위반응,동시MgSO4 14.4μg-마배역하제량0.36μg조합야감경소서통행위반응,급약후0.5h각조소서자발태족차수[(10.08±1.66)차、(7.35±1.36)차、(10.54±1.32)차]비C조[(13.05±2.06)차]강저,PWMT[ (0.81±0.22)g、(1.33±0.19)g、(0.93±0.26)g]、PWTL[ (10.57±1.53)s、(13.12±1.71)s、(11.46±1.83)s]비C조[(0.42±0.23)g、(8.87±1.27)s]승고,차이유통계학의의(P＜0.05).작용2h체도고봉,유지도4h좌우,8h후기본소실.결론 초내주사MgSO4능구제량의뢰성개선골암통소서적통행위；동시MgSO4가증강역하제량마배적진통작용.
Objective To investigate the effect of intrathecal injection of magnesium sulfate ( MgSO4 ) on pain behavior in mouse with bone cancer pain.Methods 56 male 8-10 week old C3H/HeJ mice weighing 18-22 g were divided randomly into 7 groups ( n =8 ):sham group (S group),control group (C group) and MgSO4 plus morphine treat groups( T1-T5 group).Croup C and T mice were induced bone cancer pain models by intra-rightfemur inoculation of osteolytic NCTC2472 cells while group S were injected of only α-MEM.On the 14d after inoculation,group S and C received intrathecal injection of artificial cerebrospinal fluid 5 μl,while group T1-T5 received intrathecal injection of MgSO4 14.4 μg,43.2 μg,86.4 μg,morphine 0.36 μg,MgSO4 14.4 μg-morphine 0.36 μg,which were dissolved in 5 μl artificial cerebrospinal fluid.Micc received pain behavior tests including quantification of spontaneous flinches,paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at 0.5h before and 0.5h,2h,4h,gh after administration.Results Treatment with MgSO4 (14.4 μg),morphine (0.36 μg) have no effect on bone cancer pain,while treatment with MgSO4 (43.2 μg,86.4 μg)can dose-dependently reverse quantification of spontaneous flinches,mechanical allodynia and thermal hypcralgesia which were induced by inoculation as well as MgSO4 14.4 μg-morphine 0.36 μg.At 0.5 h after administration,the quantification of spontancous flinches of the three groups( ( 10.08 ± 1.66),(7.35 ± 1.36),( 10.54 ± 1.32 ) ) were decrcased when compared with control group ( 13.05 ± 2.06 ),PWMT ( (0.81 ± 0.22 ) g; ( 1.33 ± 0.19)g; (0.93 ±0.26)g),PWTL( (10.57 ±1.53)s; (13.12 ±1.71)s; (11.46 ±1.83)s) were increased when compared with control group ( (0.42 ± 0.23 ) g,( 8.87 ± 1.27 ) s) (P ＜ 0.05 ).The effect reached maximum level at 2h,lasted for at least 4h and disappeared at 8h.Conclusion Intrathecal injection MgSO4 can effectively attenuate bone cancer pain dose-dependently.At the same time MgSO4 can amplify the analgesic effect of subliminal morphine.