CAJ | 학술논문

目的探讨冰冻血小板融化复苏后的稳定性.方法将血小板分别置于-80℃和-90℃～-120℃保存,观察冰冻血小板融化复苏后纤维蛋白及絮状聚集物的情况.结果 80℃保存血小板纤维蛋白及絮状聚集率为23%,-90℃～-120℃保存血小板纤维蛋白及絮状聚集率为0.血小板保存温度不同,纤维蛋白及絮状聚集发生率的差异有显著性(x2=219.64,P＜0.01),冰冻血小板冷冻速度和保存温度对血小板质量的影响有显著性.结论冰冻血小板于-90℃～-120℃保存的稳定性比较好.
목적 탐토빙동혈소판융화복소후적은정성.방법 장혈소판분별치우-80℃화-90℃～-120℃보존,관찰빙동혈소판융화복소후섬유단백급서상취집물적정황.결과 80℃보존혈소판섬유단백급서상취집솔위23%,-90℃～-120℃보존혈소판섬유단백급서상취집솔위0.혈소판보존온도불동,섬유단백급서상취집발생솔적차이유현저성(x2=219.64,P＜0.01),빙동혈소판냉동속도화보존온도대혈소판질량적영향유현저성.결론 빙동혈소판우-90℃～-120℃보존적은정성비교호.
Objective To study the stability of melt resuscitative frozen platelets. Methods Frozen platelets were stored in different temperature, at-80℃ and-90℃～-120℃ respectively. The formation of fibrin and irreversible aggregation in these melt resuscitative frozen platelets were observed. Results The rate of fibrin and irreversible aggregation was 23% when platelets were stored at-80℃. The rate of fibrin and irreversible aggregation was 0 when platelets were stored at-90℃～ -120℃. In different temperature the formation of fibrin and irreversible aggregation were significantly different(χ2 = 219.64, P ＜ 0.01 ). The maintain temperature and frozen velocity inplatelets could affect the quality of platelets. Conclusion Frozen platelets stored at-90℃～-120℃ have good stability ofmelt resuscitative frozen platelets by preservation the labile coagulation factors in platelets.