CAJ | 학술논문

目的用骨质疏松及牙槽骨吸收动物模型评价阿仑膦酸盐对骨吸收的防治作用。方法建立骨质疏松及牙槽骨吸收的动物模型，建模手术次日，给实验组皮下注射阿仑膦酸钠液，每周3次，共6周。用血生化指标、骨生物力学、骨密度测量及组织形态学方法进行药效评价。结果股骨密度：对照组（0.123 g/cm2）与实验组（0.151 g/cm2）相比明显降低（P<0.01）；血清碱性磷酸酶：对照组（259.63 U/L）与实验组（151.13 U/L）相比明显升高(P<0.01)；骨生物力学各项指标，实验组与对照组也有明显差异。组织形态学：实验组牙龈轻度炎症，牙槽嵴顶未见明显吸收；对照组牙龈乳突炎症明显，上皮钉突增生，炎细胞浸润，牙槽嵴顶明显吸收。结论阿仑膦酸钠能减少骨质丢失从而可以防止骨质疏松及病理性牙槽骨骨吸收。
목적용골질소송급아조골흡수동물모형평개아륜련산염대골흡수적방치작용。방법건립골질소송급아조골흡수적동물모형，건모수술차일，급실험조피하주사아륜련산납액，매주3차，공6주。용혈생화지표、골생물역학、골밀도측량급조직형태학방법진행약효평개。결과고골밀도：대조조（0.123 g/cm2）여실험조（0.151 g/cm2）상비명현강저（P<0.01）；혈청감성린산매：대조조（259.63 U/L）여실험조（151.13 U/L）상비명현승고(P<0.01)；골생물역학각항지표，실험조여대조조야유명현차이。조직형태학：실험조아간경도염증，아조척정미견명현흡수；대조조아간유돌염증명현，상피정돌증생，염세포침윤，아조척정명현흡수。결론아륜련산납능감소골질주실종이가이방지골질소송급병이성아조골골흡수。
Objective　To evaluate the anti-osteoporosis effect of alendronate on bone in rat model. Methods　The osteoporosis and alveolar bone resorption animal model were established in rats. The experimental groups were given alendronate in 5 mg/kg BW by subcutaneous injection after first day of ovariectomy and dental ligature, three times a week for 6 weeks. All the animals were sacrifised 12 weeks after the operation. The blood samples were collected for determination of biochemical indices. The left femora and jaw bones were processed for histomorphometry. The right femora and mendibles were prepared for determination of bone density and bone biodynamics. Results　As compared with the control groups(without alendronate), the values of experimental groups (with alendronate) were all significantly improved on bone density, bone anti-flexure ability and body weight. The alkaline phosphatase, calcium in serum of ovariectomy groups(without alendronate) were obviously increased. In the experimental group, however, these indices were close to normal levels. In histomorphometry, there were little inflammation in gingiva and no obvious bone resorption in the alveolar crests of the experimental groups; but there were obvious gingivitis and alveolar crests resorption of the control groups. Conclusions　Alendronate can effectively prevent bone loss to reduce osteoporosis in ovariectomized rats and prohibit pathological alveolar bone resorption.