CAJ | 학술논문

通过紫外诱变筛选得到呼吸缺陷型酿酒酵母R40，再以R40和管囊酵母P01为亲本，进行原生质体制备与再生的研究，考察了酶浓度、酶解温度和酶解时间对两亲本原生质体制备和再生的影响。结果表明，当酶解时间为1h，酶浓度为2％时，35℃下酶解酿酒酵母R40所得原生质体的形成率和再生率分别达到96．1％和12．5％；30℃下酶解管囊酵母P01所得原生质体的形成率和再生率为97．2％和10．1％。
통과자외유변사선득도호흡결함형양주효모R40，재이R40화관낭효모P01위친본，진행원생질체제비여재생적연구，고찰료매농도、매해온도화매해시간대량친본원생질체제비화재생적영향。결과표명，당매해시간위1h，매농도위2％시，35℃하매해양주효모R40소득원생질체적형성솔화재생솔분별체도96．1％화12．5％；30℃하매해관낭효모P01소득원생질체적형성솔화재생솔위97．2％화10．1％。
R40 S.cerevisiae of respiration defect type was screened out by UV mutagensis, and then the preparation and regeneration ofprotoplast with R40 S.cerevisiae and P01 Pachysolen tannophilus as parent strains were carried out and the effects of enzyme concentration, enzymatic hydrolysis temperature, and enzymatic hydrolysis time on the preparation and regeneration of protoplast were investigated. The results showed that as enzyme concentration was 2 % and enzymatic hydrolysis time was lh, the preparation rate and regeneration rate of protoplast for Angel R40 S. cerevisiae could reach up 96.1% and 12.5 % respectively at 35 ~C, and the preparation rate and regeneration rate of protoplast for P01 Pachysolen tannophilus could reach up 97.2 % and 10.1% respectively at 30 ℃.