CAJ | 학술논문

基于近红外光谱结合化学模式识别中的偏最小二乘法研究了一种快速、简单和低成本检测STR基因型的方法．选择STR基因座D5S818中的总串数相差较大的10—10、11—11与13—13基因型作为研究对象，将这三个基因型样本进行标准的PCR扩增并采集PCR产物的近红外光谱，以每一基因型的任意三分之二样本作为校正集，剩余三分之一作为预测集样本，探索了基于近红外光谱进行基因分型的可能性，结果发现该三类模型能够得到正确的判别，没有误判，预测集预测率达到100％．成功实现了基于近红外光谱对STR基因型的快速、简单和低成本检测．
기우근홍외광보결합화학모식식별중적편최소이승법연구료일충쾌속、간단화저성본검측STR기인형적방법．선택STR기인좌D5S818중적총천수상차교대적10—10、11—11여13—13기인형작위연구대상，장저삼개기인형양본진행표준적PCR확증병채집PCR산물적근홍외광보，이매일기인형적임의삼분지이양본작위교정집，잉여삼분지일작위예측집양본，탐색료기우근홍외광보진행기인분형적가능성，결과발현해삼류모형능구득도정학적판별，몰유오판，예측집예측솔체도100％．성공실현료기우근홍외광보대STR기인형적쾌속、간단화저성본검측．
This paper has established a method which could be used to detect short tandem repeat （STR） rapidly, simply and low-costly based on near infrared （NIR） spectroscopy combined with discriminant partial least squares （DPLS） regression. Genotypes 10-10, 11-11 and 13-13 of STR locus D5S818 were selected as the objective subjects. Through optimal polymerase chain reaction and NIR spectrum measurement, NIR spectra of samples for each genotype were obtained. The two thirds of each genotype＇s NIR spectra were used to establish DPLS genotyping models. It was shown that models had good robustness and strong discriminating power for the prediction samples （the accuracy was up to 100%）. Based on NIR spectra, this paper achieves the detection of STR genotypes in a rapid, simple and low-cost way.