CAJ | 학술논문

本研究利用生物信息学分析日本血吸虫编码Akt蛋白的cDNA并对编码该蛋白的cDNA进行了克隆和原核表达。生物信息学分析表明日本血吸虫存在两个Akt蛋白。利用PCR将其中一个Akt蛋白催化功能域的编码cDNA进行了克隆，并利用原核表达系统对此蛋白片段进行诱导表达并进行了重组蛋白的纯化，将重组蛋白免疫新西兰大白兔制备了多克隆抗体。Western blot分析表明，该抗体能被日本血吸虫Akt重组蛋白特异性识别。本研究为进一步研究该蛋白的功能奠定了基础。
본연구이용생물신식학분석일본혈흡충편마Akt단백적cDNA병대편마해단백적cDNA진행료극륭화원핵표체。생물신식학분석표명일본혈흡충존재량개Akt단백。이용PCR장기중일개Akt단백최화공능역적편마cDNA진행료극륭，병이용원핵표체계통대차단백편단진행유도표체병진행료중조단백적순화，장중조단백면역신서란대백토제비료다극륭항체。Western blot분석표명，해항체능피일본혈흡충Akt중조단백특이성식별。본연구위진일보연구해단백적공능전정료기출。
In the study, we employed bioinformatics and molecular techniques to preliminary study the cDNAs encoding for Akt proteins in Schistosoma japonicum. Bioinformatical analysis indicated that Schistosoma japonicum has two Akt proteins. Subsequently, the cDNA fragment encoding the catalytic domain of a Akt protein was amplified by PCR technique and then cloned into a expression vector pET28a（＋） . The expressed recombinant protein was purified by using a Nickel charged affinity column. The polyclonal antibodies against the Akt protein were raised by immunizing the purified protein in Newland rabbit. Western blot analysis indicated that the polyclonal antibodies were able to specifically recognize the recombinant SjAkt protein. Overall, the present study provides fundamental information for further investigating the functions of Akt proteins in Schistosomajaponicum.