CAJ | 학술논문

采用定性检测、光谱分析等方法对叶上花总黄酮的种类与提取方法进行了研究。结果显示叶上花所含黄酮种类较多、含量较高;各因素对总黄酮提取率影响程度大小顺序为温度〉乙醇体积分数〉固液比〉时间。最佳提取条件为温度60℃、乙醇体积分数70%、固液比1∶100（g/mL）、提取时间180min,在此提取条件下,总黄酮含量为1.479%。
채용정성검측、광보분석등방법대협상화총황동적충류여제취방법진행료연구。결과현시협상화소함황동충류교다、함량교고;각인소대총황동제취솔영향정도대소순서위온도〉을순체적분수〉고액비〉시간。최가제취조건위온도60℃、을순체적분수70%、고액비1∶100（g/mL）、제취시간180min,재차제취조건하,총황동함량위1.479%。
With the methods of the chemical qualitative detection and the spectral analysis,the categories of the total flavonoids in the whole plant of H.himalaica was researched,and effect of four factors（volume fraction of ethanol,temperature,ratio of solid-liquid,extraction time） on the efficiency of extraction of the total flavonoids was also evaluated by orthogonal design L9（34）.The results suggested that the total flavonoids consisted of flavones,flavonols,flavanones,flavanonols.The influencing degree of each factor on the efficiency was as follows： temperaturevolume fraction of ethanolratio of solid-liquidextraction time.The optimum extracting conditions are A1C3B2D3.The highest efficiency of extraction of the total flavonoids reached 1.479% under such optimal conditions.