基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
Genomics and Applied Biology
2011年
1期
57-61
,共5页
罗宝丽%蒋运生%唐辉%韦记青%史艳财%邹蓉%韦霄
囉寶麗%蔣運生%唐輝%韋記青%史豔財%鄒蓉%韋霄
라보려%장운생%당휘%위기청%사염재%추용%위소
块根紫金牛%总黄酮%提取工艺%含量测定
塊根紫金牛%總黃酮%提取工藝%含量測定
괴근자금우%총황동%제취공예%함량측정
Ardisiacorymbifera Mez var. tuberifera C. Chen%Flavonoid%Extracting condition%Quantity determination
本研究采用正交试验分析和分光光度法,建立了块根紫金牛总黄酮含量测定的方法并比较分析其不同产地及不同部位总黄酮的含量差异。结果表明超声波法提取块根紫金牛中总黄酮的最佳提取工艺为：乙醇浓度60%,料液比1：70,提取时间50min,提取功率200W,提取温度70℃。另外,不同产地块根紫金牛中总黄酮含量顺序为：靖西〉那坡〉德保〉扶绥〉龙州〉武鸣;同一产地块根紫金牛根、茎、叶间总黄酮含量的高低顺序大致为：叶〉根〉茎。且通过精密度、重复性、稳定性和回收率实验来检测最佳提取工艺条件,结果显示该方法操作简便,结果准确,重复性好,RSD为0.788%,稳定性和回收率较高,平均回收率达96.20%。证明该方法可用于比较不同产地块根紫金牛药材中总黄酮的含量差异,对不同产地块根紫金牛的药材质量分析具有一定的指导作用。
本研究採用正交試驗分析和分光光度法,建立瞭塊根紫金牛總黃酮含量測定的方法併比較分析其不同產地及不同部位總黃酮的含量差異。結果錶明超聲波法提取塊根紫金牛中總黃酮的最佳提取工藝為：乙醇濃度60%,料液比1：70,提取時間50min,提取功率200W,提取溫度70℃。另外,不同產地塊根紫金牛中總黃酮含量順序為：靖西〉那坡〉德保〉扶綏〉龍州〉武鳴;同一產地塊根紫金牛根、莖、葉間總黃酮含量的高低順序大緻為：葉〉根〉莖。且通過精密度、重複性、穩定性和迴收率實驗來檢測最佳提取工藝條件,結果顯示該方法操作簡便,結果準確,重複性好,RSD為0.788%,穩定性和迴收率較高,平均迴收率達96.20%。證明該方法可用于比較不同產地塊根紫金牛藥材中總黃酮的含量差異,對不同產地塊根紫金牛的藥材質量分析具有一定的指導作用。
본연구채용정교시험분석화분광광도법,건립료괴근자금우총황동함량측정적방법병비교분석기불동산지급불동부위총황동적함량차이。결과표명초성파법제취괴근자금우중총황동적최가제취공예위：을순농도60%,료액비1：70,제취시간50min,제취공솔200W,제취온도70℃。령외,불동산지괴근자금우중총황동함량순서위：정서〉나파〉덕보〉부수〉룡주〉무명;동일산지괴근자금우근、경、협간총황동함량적고저순서대치위：협〉근〉경。차통과정밀도、중복성、은정성화회수솔실험래검측최가제취공예조건,결과현시해방법조작간편,결과준학,중복성호,RSD위0.788%,은정성화회수솔교고,평균회수솔체96.20%。증명해방법가용우비교불동산지괴근자금우약재중총황동적함량차이,대불동산지괴근자금우적약재질량분석구유일정적지도작용。
In this research, we established a method of quantity determination of total flavonoid in Ardisiacorymbifera Mez var. tuberifera C. Chen and comparative analysed content differences of flavonoid in different sections A. var. tuberifera from different areas by orthogonal experiment and spectrophotometry. The results indicated the optimum extractive technology of determination of total flavonoid in A. var. tuberifera by spectrophotometry was that extraction reagent 60% alcoho1, ratio of liquid/solid as 1：70, extracting power 200 W, extracting temperature 70℃ and extracting time 50 min. On the other hand, the quantitative order of flavonoid of A. var. tuberifera from different areas was that JingxiNapoDebaoFusuiLongzhouWuming as well as the quantitative order of flavonoid in different sections of A. var. tuberifera from the same area was leafrootstem. The optimum extract condition was detected by the experiment of precision, repeatability, stability and recovery, The results displayed that this method was simple, accurate, repeatable, the RSD was 0.788%, stable and high recovery with the average recovery of 96.20%, which showed that this method was feasibling for comparative content differences of flavonoid in different sections A. var. tuberifera from different areas and would provide some important direction for its quality analysis.
