甲泼尼龙对慢性咳嗽患者诱导痰巨噬细胞中Toll样受体2表达的影响
갑발니룡대만성해수환자유도담거서세포중Toll양수체2표체적영향
Methylprednisolone enhances the expression of Toil-like receptor 2 on macrophages in induced sputum from patients with chronic cough
  • 万方数据
    中华结核和呼吸杂志 중화결핵화호흡잡지
    Chinese Journal of Tuberculosis and Respiratory Diseases
    2011年 2期 123-127 ,共5页

    汤长文%罗丽%王鹏%陈菊屏%王孝芸%李国平

    탕장문%라려%왕붕%진국병%왕효예%리국평

    咳嗽%巨噬细胞%Toll样受体2%甲泼尼龙 咳嗽%巨噬細胞%Toll樣受體2%甲潑尼龍
    해수%거서세포%Toll양수체2%갑발니룡
    Cough%Macrophages%Toll-like receptor 2%Methylprednisolone
    目的 探讨Toll样受体2(TLR2)在慢性咳嗽患者诱导痰巨噬细胞中的表达与气道炎症的关系以及甲泼尼龙对TLR2表达的影响.方法 收集2008年9月至2010年3月泸州医学院附属医院呼吸科门诊的慢性咳嗽患者86例,其中咳嗽变异性哮喘(CVA)组31例,上气道咳嗽综合征(UACS)组14例,嗜酸细胞性支气管炎(EB)组16例,胃食管反流性咳嗽(GERC)组25例.对照组为20名健康志愿者.各组均行痰液诱导检查,HE染色,免疫荧光染色测定TLR2表达,酶联免疫吸附法(ELISA)测定诱导痰上清液中细胞因子的浓度.结果 (1)CVA、UACS、EB和GERC各组患者诱导痰中细胞总数[分别为(3.4±1.1)×106/L、(2.6±0.6)×106/L、(2.6±0.6)×106/L、(2.7±0.5)×106/L]均明显高于对照组[(1.4±0.6)×106/L],差异均有统计学意义(均P＜0.01);分类计数显示以上4组痰中巨噬细胞比例(分别为0.35±0.04、0.40±0.06、0.38±0.04、0.43±0.05)均明显低于对照组(0.63±0.08);中性粒细胞比例(分别为0.52±0.06、0.58±0.06、0.52±0.04、0.54±0.06)明显高于对照组,差异均有统计学意义(均P＜0.01).CVA和EB组患者嗜酸细胞比例(分别为0.115±0.054和0.099±0.034)明显高于其他各组,差异有统计学意义(均P＜0.01),但两组间差异无统计学意义(t=1.18,P＞0.05).(2)CVA、UACS、EB和GERC各组患者诱导痰上清液白细胞介素-8浓度[分别为(7.0±1.2)μg/L、(10.2±3.1)μg/L、(6.3±1.9)μg/L、(7.7±2.5)μg/L]和肿瘤坏死因子-α浓度[分别为(30±11)ng/L、(46±16)ng/L、(2l±5)ng/L、(33±15)ng/L]明显高于对照组[分别为(1.6±0.8)μg/L和(13±6)ng/L],差异均有统计学意义(均P＜0.01).(3)CVA、UACS、EB和GERC各组患者诱导痰上清液中白细胞介素-8与痰中中性粒细胞数呈正相关(r值分别为0.628、0.816、0.769、0.733,均P＜0.05);CVA、UACS、EB和GERC各组患者诱导痰上清液中肿瘤坏死因子-α与痰中中性粒细胞数呈正相关(r值分别为0.579、0.865、0.730、0.755,均P＜0.05).(4)CVA、UACS、EB和GERC各组患者诱导痰巨噬细胞TLR2表达量(56±15、38±7、65±17、27±4)明显低于对照组(135±14),甲泼尼龙可上调以上各组患者诱导痰巨噬细胞中TLR2(75±17、53.29±16、94±30、41±5)的表达.结论 CVA、UACS、EB和GERC等慢性咳嗽患者诱导痰巨噬细胞中TLR2的表达下降,甲泼尼龙可上调巨噬细胞TLR2的表达.
    목적 탐토Toll양수체2(TLR2)재만성해수환자유도담거서세포중적표체여기도염증적관계이급갑발니룡대TLR2표체적영향.방법 수집2008년9월지2010년3월로주의학원부속의원호흡과문진적만성해수환자86례,기중해수변이성효천(CVA)조31례,상기도해수종합정(UACS)조14례,기산세포성지기관염(EB)조16례,위식관반류성해수(GERC)조25례.대조조위20명건강지원자.각조균행담액유도검사,HE염색,면역형광염색측정TLR2표체,매련면역흡부법(ELISA)측정유도담상청액중세포인자적농도.결과 (1)CVA、UACS、EB화GERC각조환자유도담중세포총수[분별위(3.4±1.1)×106/L、(2.6±0.6)×106/L、(2.6±0.6)×106/L、(2.7±0.5)×106/L]균명현고우대조조[(1.4±0.6)×106/L],차이균유통계학의의(균P＜0.01);분류계수현시이상4조담중거서세포비례(분별위0.35±0.04、0.40±0.06、0.38±0.04、0.43±0.05)균명현저우대조조(0.63±0.08);중성립세포비례(분별위0.52±0.06、0.58±0.06、0.52±0.04、0.54±0.06)명현고우대조조,차이균유통계학의의(균P＜0.01).CVA화EB조환자기산세포비례(분별위0.115±0.054화0.099±0.034)명현고우기타각조,차이유통계학의의(균P＜0.01),단량조간차이무통계학의의(t=1.18,P＞0.05).(2)CVA、UACS、EB화GERC각조환자유도담상청액백세포개소-8농도[분별위(7.0±1.2)μg/L、(10.2±3.1)μg/L、(6.3±1.9)μg/L、(7.7±2.5)μg/L]화종류배사인자-α농도[분별위(30±11)ng/L、(46±16)ng/L、(2l±5)ng/L、(33±15)ng/L]명현고우대조조[분별위(1.6±0.8)μg/L화(13±6)ng/L],차이균유통계학의의(균P＜0.01).(3)CVA、UACS、EB화GERC각조환자유도담상청액중백세포개소-8여담중중성립세포수정정상관(r치분별위0.628、0.816、0.769、0.733,균P＜0.05);CVA、UACS、EB화GERC각조환자유도담상청액중종류배사인자-α여담중중성립세포수정정상관(r치분별위0.579、0.865、0.730、0.755,균P＜0.05).(4)CVA、UACS、EB화GERC각조환자유도담거서세포TLR2표체량(56±15、38±7、65±17、27±4)명현저우대조조(135±14),갑발니룡가상조이상각조환자유도담거서세포중TLR2(75±17、53.29±16、94±30、41±5)적표체.결론 CVA、UACS、EB화GERC등만성해수환자유도담거서세포중TLR2적표체하강,갑발니룡가상조거서세포TLR2적표체.
    Objective To explore the relationship between airway inflammation and Toll-like receptor 2 ( TLR2 ) expression on macrophages in induced sputum from chronic cough patients, and to observe the effect of methylprednisolone on this receptor. Methods Eighty-six outpatients with chronic cough were enrolled in this study from the respiratory department in the Affiliated Hospital of Luzhou Medical College from September 2008 to March 2010. These diseases were diagnosed according to the guideline of Chinese Society of Respiratory Diseases in 2005, excluding severe liver, heart and kidney pathology and pregnant women. Among 86 cases, 31 were cough variant asthma ( CVA), 14 upper airway cough syndrome (UACS), 16 eosinophilic bronchitis (EB) and 25 gastroesophageal reflux (GERC). Twenty healthy volunteers were included as controls. Induced sputum was collected and cell counts were analyzed. TLR2 expression on macrophages was determined by immunofluorescence staining. Cytokine levels were measured with enzyme linked immunosorbent assay (ELISA). Results (1) The total inflammatory cells [(3.4±1.1) ×106/L, (2.6 ±0.6)×106/L, (2.6±0.6)×106/L, (2.7±0.5) × 106/L] and neutrophils (0.52±0.06, 0.58 ±0.06, 0.52±0.04, 0.54 ±0.06) in induced sputum from CVA, UACS, EB and GERC were significantly increased(t value = 5.27, 4.09, 3. 16, 3.92 and 3.62, 3.49, 4.82, 6.17respectively, all P＜0.01 ), with decreased macrophages (0.35±0.04, 0.40±0.06,0.38±0.04,0.43 ±0. 05) in comparison with the control group (t value =4. 76,5.24,4. 57,3. 18, all P ＜0. 01 ). There was a significant increase in eosinophils(0.118±0.054,0.099±0.034) in CVA and EB group (t value =4.46,3.87,5. 19 and 3.51,4. 06,4. 38 respectively, all P＜0.01). (2) The concentration of IL-8[(7.0±1.2)μg/L,(10.2±3.1)μg/L,(6.3±1.9)μg/L,(7.7±2.5)μg/L] and TNF-α[(30 ± 11)ng/L, (46±16)rng/L, (21 ±5)ng/L, (33 ± 15)ng/L] from induced sputum in CVA, UACS, EB and GERC groups were significantly increased, compared with the control group( t value =4. 58,3.67,4. 26,5. 39 and 5.16,3.97,4. 59,3.34 respectively, all P ＜ 0.01 ). (3) The concentration of IL-8 was positively correlated with neutrophils in each group( R value = 0.628,0. 816,0. 769 and 0. 733 respectively, all P＜0. 05 ). The concentration of TNF-o was also positively correlated with neutrophils in each group ( R value = 0. 579,0. 865,0. 730 and 0. 755 respectively,all P ＜ 0. 05 ). (4) The expression of TLR2 ( 56 ± 15,38 ± 7,65 ±17,27 ±4) on macrophages in CVA, UACS,EB and GERC was significantly decreased. The expression of TLR2 (75±17, 53±16,94±30,41±5 ) on macrophages was enhanced by methylprednisolone.Conclusions The expression of TLR2 on macrophages from induced sputum in CVA, UACS, EB and GERC was decreased, but could be enhanced by methylprednisolone, suggesting that TLR2 might play a regulatory role in airway inflammatory response.
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