CAJ | 학술논문

目的探讨炎性趋化因子MCP-1及致纤维化因子TGF-β1在胆道梗阻早期大鼠中的表达及与肝脏损伤相应指标的关系.方法 50只Wistar雄性大鼠随机分为3组:正常组、假手术组和胆道梗阻组.术后10 d,各组大鼠检测血清ALT和BIL-T值,肝脏匀浆组织测得MDA含量,酶联免疫吸附试验反应血清TGF-β1指标,免疫组化表达肝脏MCP-1的浸润程度.结果相比正常组及假手术组大鼠的各检测指标,早期胆道梗阻组大鼠肝脏MDA含量、MCP-1表达强度及血清ALT、BIL-T、TGF-β1水平均升高.胆道梗阻早期大鼠肝脏MCP-1表达与血清ALT及BIL-T升高有关,MCP-1高表达还与肝脏氧化应激指标MDA含量增加有关.血清TGF-β1水平升高只与血清ALT及BIL-T数值增加相关.而与MDA含量增加无关.结论胆道梗阻早期大鼠肝脏MCP-1高表达及血清TGF-β1指标升高均与肝脏损伤及胆汁淤积有关,同时MCP-1的高表达还与肝脏氧化应激一致,二者对胆道梗阻早期肝脏损伤机制研究有重要意义.
목적 탐토염성추화인자MCP-1급치섬유화인자TGF-β1재담도경조조기대서중적표체급여간장손상상응지표적관계.방법 50지Wistar웅성대서수궤분위3조:정상조、가수술조화담도경조조.술후10 d,각조대서검측혈청ALT화BIL-T치,간장균장조직측득MDA함량,매련면역흡부시험반응혈청TGF-β1지표,면역조화표체간장MCP-1적침윤정도.결과 상비정상조급가수술조대서적각검측지표,조기담도경조조대서간장MDA함량、MCP-1표체강도급혈청ALT、BIL-T、TGF-β1수평균승고.담도경조조기대서간장MCP-1표체여혈청ALT급BIL-T승고유관,MCP-1고표체환여간장양화응격지표MDA함량증가유관.혈청TGF-β1수평승고지여혈청ALT급BIL-T수치증가상관.이여MDA함량증가무관.결론 담도경조조기대서간장MCP-1고표체급혈청TGF-β1지표승고균여간장손상급담즙어적유관,동시MCP-1적고표체환여간장양화응격일치,이자대담도경조조기간장손상궤제연구유중요의의.
Objective To investigate the expression of MCP-1 and TGF-β1 in early stage of ob-structive jaundice and explore its relation to liver correlated injury indexes in rats. Methods Fifty male Wistar rats were randomized into the control group, sham-operated group and biliary obstruction group. On day 10, serum ALT and BIL-T levels were determined in inferior caval blood and hepatic MDA was estimated in liver homogenates. Meanwhile, serum TGF-β1 level was measured by ELISA and the MCP-1 infiltration determined with immunohistochemistry. Results Serum ALT and BIL-T values were elevated. Meanwhile, the liver MDA content was increased. The positive expression of MCP-1 was augmented and serum TGF-β1 was over-expressed in the early stage after obstructive jaun-dice in rats. The hepatic MCP-1 expression had a positive correlation with the elevated ALT, BIL-T and MDA levels. However, the serum TGF-β1 content only had a positive correlation with ALT and BIL-T. Conclution Hepatic MCP-1 and serum TGF-β1 expression in the early stage after biliary tract obstruction is related to liver injury and extrahepatic cholestasis. Meanwhile, the hepatic MCP-1 ex-pression is correlated with hepatic oxidation stress. They have an important significance in liver injury in rats during the early period of obstructive jaundice.