中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2009年
1期
41-43
,共3页
李群伟%侯海峰%李晓梅%李亚鲁%韩纪举%王欣农
李群偉%侯海峰%李曉梅%李亞魯%韓紀舉%王訢農
리군위%후해봉%리효매%리아로%한기거%왕흔농
软骨,关节%脱氧雪腐镰刀菌烯醇%病因学
軟骨,關節%脫氧雪腐鐮刀菌烯醇%病因學
연골,관절%탈양설부렴도균희순%병인학
Cartilage,articular%Deoxynivalenol%Etiology
目的 观察脱氧雪腐镰刀菌烯醇(DON)对新西兰家兔膝关节软骨和滑膜的损伤.方法 将15只成年雄性新西兰家兔随机分为3组,对照组正常饲养,高、低剂量组分别投予0.10、0.05 mg/kg剂量的DON,经耳缘静脉注射隔日染毒.20 d后处死家兔,取双侧膝关节,进行常规病理检查,测定关节冲洗液白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、一氧化氮(NO)水平.结果 光镜下,可见低、高剂量组家兔膝关节软骨细胞变性、坏死.滑膜组织炎细胞浸润.关节冲洗液IL-1β、TNF-α、NO,组间比较差异有统计学意义(F值分别为19.396、18.195、22.136,P＜0.05);与对照组[(0.113±0.043)、(0.138±0.087)μg/L、(23.56±9.35)μmol/L]比较,高剂量组[(0.451±0.091)、(0.575±0.122)μg/L,(70.27±11.53)μmol/L]和低剂量组[(0.295±0.107)、(0.387±0.131)μg/L,(45.32±12.24)μmol/L]明显增高(P＜0.05),且高剂量组高于低剂量组(P＜0.05).结论 DON可导致家兔关节软骨和滑膜损伤,其损伤类似于人骨关节炎病变.DON可能是骨关节炎的病因之一.
目的 觀察脫氧雪腐鐮刀菌烯醇(DON)對新西蘭傢兔膝關節軟骨和滑膜的損傷.方法 將15隻成年雄性新西蘭傢兔隨機分為3組,對照組正常飼養,高、低劑量組分彆投予0.10、0.05 mg/kg劑量的DON,經耳緣靜脈註射隔日染毒.20 d後處死傢兔,取雙側膝關節,進行常規病理檢查,測定關節遲洗液白細胞介素1β(IL-1β)、腫瘤壞死因子α(TNF-α)、一氧化氮(NO)水平.結果 光鏡下,可見低、高劑量組傢兔膝關節軟骨細胞變性、壞死.滑膜組織炎細胞浸潤.關節遲洗液IL-1β、TNF-α、NO,組間比較差異有統計學意義(F值分彆為19.396、18.195、22.136,P＜0.05);與對照組[(0.113±0.043)、(0.138±0.087)μg/L、(23.56±9.35)μmol/L]比較,高劑量組[(0.451±0.091)、(0.575±0.122)μg/L,(70.27±11.53)μmol/L]和低劑量組[(0.295±0.107)、(0.387±0.131)μg/L,(45.32±12.24)μmol/L]明顯增高(P＜0.05),且高劑量組高于低劑量組(P＜0.05).結論 DON可導緻傢兔關節軟骨和滑膜損傷,其損傷類似于人骨關節炎病變.DON可能是骨關節炎的病因之一.
목적 관찰탈양설부렴도균희순(DON)대신서란가토슬관절연골화활막적손상.방법 장15지성년웅성신서란가토수궤분위3조,대조조정상사양,고、저제량조분별투여0.10、0.05 mg/kg제량적DON,경이연정맥주사격일염독.20 d후처사가토,취쌍측슬관절,진행상규병리검사,측정관절충세액백세포개소1β(IL-1β)、종류배사인자α(TNF-α)、일양화담(NO)수평.결과 광경하,가견저、고제량조가토슬관절연골세포변성、배사.활막조직염세포침윤.관절충세액IL-1β、TNF-α、NO,조간비교차이유통계학의의(F치분별위19.396、18.195、22.136,P＜0.05);여대조조[(0.113±0.043)、(0.138±0.087)μg/L、(23.56±9.35)μmol/L]비교,고제량조[(0.451±0.091)、(0.575±0.122)μg/L,(70.27±11.53)μmol/L]화저제량조[(0.295±0.107)、(0.387±0.131)μg/L,(45.32±12.24)μmol/L]명현증고(P＜0.05),차고제량조고우저제량조(P＜0.05).결론 DON가도치가토관절연골화활막손상,기손상유사우인골관절염병변.DON가능시골관절염적병인지일.
Objective To observe toxic effect of deoxynivalenol(DON)on articular cartilage and synovium of New Zealand rabbits's knee ioints.Methods Fifteen male rabbits were divided randomly into 3 groups:control, high-dosage,and low-dosage group.In high-dosage and low-dosage group,saline solution of DON was injected with a dose of 0.10 and 0.05 ms/kg every 48 h into ear vein of rabbits.Specimen of articular cartilage and synovium were through pathologY methods,and IL-1β,TNF-α,NO levels were assayed in joint liquid,after 20 days. Results Morphological changes were observed, such as synovium inflammative infiltration, chondrocytes deformation and necrosis under light microscope.The levels of IL-1β,TNF-α and NO had statistical significance in comDarison between 3 grouPs(F=19.396,18.195,22.136,P＜0.05).The levels of IL-1β,TNF-α and NO were significantly higher(all P＜0.05),high-dosage[(0.451±0.091),(0.575±0.122)μg/L;(70.27±11.53)μmol/L] and low-dosage group[(0.295±0.107),(0.387±0.131)μg/L;(45.32±12.24)μmol/L]compared with control ((0.1 13±0.049),(0.138±0.087)μg/L;(23.56±9.35)μmoL/L],and high-dosage compared with low-dosage group Conclusions DON results in articular and synovial impairment,which has the symptom similar to osteoarthritis. DON probably causes osteoarthritis.
