中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2012年
1期
51-54
,共4页
王伟%王治伦%陈静宏%于伯泉%杨占田%马天有
王偉%王治倫%陳靜宏%于伯泉%楊佔田%馬天有
왕위%왕치륜%진정굉%우백천%양점전%마천유
活性氧%大骨节病%抗氧化酶%硒
活性氧%大骨節病%抗氧化酶%硒
활성양%대골절병%항양화매%서
Reactive oxygen species%KaschinBeck disease%Peroxidase%Selenium
目的 观察青海省兴海县大骨节病(KBD)病区儿童血清中丙二醛(MDA)及抗氧化酶的变化,探讨体内氧自由基损伤与KBD的关系.方法 在青海省兴海县KBD病区唐乃亥乡小学、下鹿圈村小学以及曲什安乡小学选择7～ 12岁的KBD患儿64名作为KBD组,46名健康儿童作为内对照组,在非病区陕西省长安区南樊小学选择59名健康儿童作为外对照组.分别采集其枕部发样和清晨空腹外周血,采用单纯随机抽样方法在每组中各抽取20例发样及血样,应用2,3-二告氨基萘(DAN)荧光法测定发硒和血硒水平；通过生化方法检测样本血清中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力,总抗氧化能力(T-AOC)及MDA水平.结果 KBD组发硒[(67.64±17.28)μg/L]、血硒[(36.27±13.29)μg/L]和GSH-Px活力[ (59.53±25.23)kU/L]明显低于内对照组[(153.32±24.31)、(63.06±13.66) μg/L,(91.88±22.99)kU/L]和外对照组[ (242.35±38.56)、(98.93±17.18)μg/L,( 122.68±41.74)kU/L],且内对照组明显低于外对照组(P均＜ 0.05)；KBD组和内对照组SOD活力[(55.80±8.14)、(57.45±6.96)kU/L]、CAT活力[(16.45±5.61)、( 15.63±9.18)kU/L]和T-AOC水平[(19.80±6.64)、(21.71±8.82)kU/L]均高于外对照组[(42.79±8.10)、(6.05±2.71)、(13.56±5.38)kU/L,P均＜0.05]；KBD组MDA水平[(4.64±1.11 )μmol/L]高于内对照组[(3.31±1.22) μmol/L]和外对照组[(3.43±1.29) μmol/L,P均＜0.05].结论 KBD存在氧化应激损伤,抗氧化酶在KBD病区儿童血清中升高是一种代偿性反应,体内氧自由基损伤与KBD的发生发展可能有一定的关系.
目的 觀察青海省興海縣大骨節病(KBD)病區兒童血清中丙二醛(MDA)及抗氧化酶的變化,探討體內氧自由基損傷與KBD的關繫.方法 在青海省興海縣KBD病區唐迺亥鄉小學、下鹿圈村小學以及麯什安鄉小學選擇7～ 12歲的KBD患兒64名作為KBD組,46名健康兒童作為內對照組,在非病區陝西省長安區南樊小學選擇59名健康兒童作為外對照組.分彆採集其枕部髮樣和清晨空腹外週血,採用單純隨機抽樣方法在每組中各抽取20例髮樣及血樣,應用2,3-二告氨基萘(DAN)熒光法測定髮硒和血硒水平；通過生化方法檢測樣本血清中穀胱甘肽過氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、過氧化氫酶(CAT)活力,總抗氧化能力(T-AOC)及MDA水平.結果 KBD組髮硒[(67.64±17.28)μg/L]、血硒[(36.27±13.29)μg/L]和GSH-Px活力[ (59.53±25.23)kU/L]明顯低于內對照組[(153.32±24.31)、(63.06±13.66) μg/L,(91.88±22.99)kU/L]和外對照組[ (242.35±38.56)、(98.93±17.18)μg/L,( 122.68±41.74)kU/L],且內對照組明顯低于外對照組(P均＜ 0.05)；KBD組和內對照組SOD活力[(55.80±8.14)、(57.45±6.96)kU/L]、CAT活力[(16.45±5.61)、( 15.63±9.18)kU/L]和T-AOC水平[(19.80±6.64)、(21.71±8.82)kU/L]均高于外對照組[(42.79±8.10)、(6.05±2.71)、(13.56±5.38)kU/L,P均＜0.05]；KBD組MDA水平[(4.64±1.11 )μmol/L]高于內對照組[(3.31±1.22) μmol/L]和外對照組[(3.43±1.29) μmol/L,P均＜0.05].結論 KBD存在氧化應激損傷,抗氧化酶在KBD病區兒童血清中升高是一種代償性反應,體內氧自由基損傷與KBD的髮生髮展可能有一定的關繫.
목적 관찰청해성흥해현대골절병(KBD)병구인동혈청중병이철(MDA)급항양화매적변화,탐토체내양자유기손상여KBD적관계.방법 재청해성흥해현KBD병구당내해향소학、하록권촌소학이급곡십안향소학선택7～ 12세적KBD환인64명작위KBD조,46명건강인동작위내대조조,재비병구합서성장안구남번소학선택59명건강인동작위외대조조.분별채집기침부발양화청신공복외주혈,채용단순수궤추양방법재매조중각추취20례발양급혈양,응용2,3-이고안기내(DAN)형광법측정발서화혈서수평；통과생화방법검측양본혈청중곡광감태과양화물매(GSH-Px)、초양화물기화매(SOD)、과양화경매(CAT)활력,총항양화능력(T-AOC)급MDA수평.결과 KBD조발서[(67.64±17.28)μg/L]、혈서[(36.27±13.29)μg/L]화GSH-Px활력[ (59.53±25.23)kU/L]명현저우내대조조[(153.32±24.31)、(63.06±13.66) μg/L,(91.88±22.99)kU/L]화외대조조[ (242.35±38.56)、(98.93±17.18)μg/L,( 122.68±41.74)kU/L],차내대조조명현저우외대조조(P균＜ 0.05)；KBD조화내대조조SOD활력[(55.80±8.14)、(57.45±6.96)kU/L]、CAT활력[(16.45±5.61)、( 15.63±9.18)kU/L]화T-AOC수평[(19.80±6.64)、(21.71±8.82)kU/L]균고우외대조조[(42.79±8.10)、(6.05±2.71)、(13.56±5.38)kU/L,P균＜0.05]；KBD조MDA수평[(4.64±1.11 )μmol/L]고우내대조조[(3.31±1.22) μmol/L]화외대조조[(3.43±1.29) μmol/L,P균＜0.05].결론 KBD존재양화응격손상,항양화매재KBD병구인동혈청중승고시일충대상성반응,체내양자유기손상여KBD적발생발전가능유일정적관계.
Objective To observe the activities of serum peroxidase capacity,and lipid peroxidation of children from Kaschin-Beck disease (KBD) areas of Xinghai county in Qinhai province,and to explore the relationship between antioxidant capacity and KBD.Methods Sixty four KBD and forty six health subjects without KBD were chosen from KBD endemic areas,which included primary schools of Tangnaihai,Xialujuan and Qushian of Xinghai county in Qinghai province,and fifty nine age-matched healthy control subjects without KBD were from a non-KBD endemic area,Nanfan primary school of Chang'an county in Shaanxi province.Twenty patients with KBD and twenty control subjects from KBD areas and non-KBD area were extracted by simple random sampling method.2,3-DAN fluorescence technique was used to test the hair and blood selenium.The biochemical techniques were used to test the indicators of oxidative stress including malondialdehyde(MDA),antioxidant enzyme activities,total antioxidant capacity(T-AOC),serum superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSHPx).Results All patients with KBD had significantly lower serum GSH-Px activities[ (59.53 ± 25.23)kU/L] and selenium levels in hair[ (67.64 ± 17.28)μg/L] and blood[(36.27 ± 13.29)μg/L],respectively,than that of control subjects from KBD areas [ ( 91.88 ± 22.99 ) kU/L,( 153.32 ± 24.31 ) μg/L,( 63.06 ± 13.66) μg/L ] and nonKBD areas[ ( 122.68 ± 41.74)kU/L,(242.35 ± 38.56)μg/L,(98.93 ± 17.18)μg/L,all P ＜ 0.05].Serum MDA levels in KBD patients[ (4.64 ± 1.11 )μmol/L] were significantly higher than that in control subjects from KBD [(3.31 ± 1.22)μmol/L] and non-KBD areas[ (3.43 ± 1.29)μmol/L,all P ＜ 0.05].On the other hand,T-AOC,SOD and CAT activities were significantly higher in both KBD[(19.80 ± 6.64),(55.80 ± 8.14),(16.45 ± 5.61 ) kU/L] and control subjects[ (21.71 ± 8.82),(57.45 ± 6.96),(15.63 ± 9.18)kU/L] from KBD areas than that of control subjects from non-KBD area[ (13.56 ± 5.38),(42.79 ± 8.10),(6.05 ± 2.71 )kU/L,all P ＜ 0.05 ].Hair selenium levels,blood selenium levels and GSH-Px activity of control subjects from KBD areas were,respectively,significantly lower than that in control subjects from non-KBD area(all P ＜ 0.05).Conclusions These findings strongly confirm the evidence that KBD patients are susceptible to oxidative stress.The results also show the increase in antioxidant enzymes,which could probably be due to adaptive response to pro-oxidant in KBD state.Hence,there seems to be an imbalance between oxidant and antioxidant systems in KBD patients.
