CAJ | 학술논문

目的检测RECK蛋白及膜型基质金属蛋白酶MMP-14在神经母细胞瘤中的表达,探讨其在神经母细胞瘤的发生、发展、浸润和转移中的作用.方法选择临床及病理资料齐全的存档神经母细胞瘤蜡块标本36例,另取节细胞神经瘤蜡块标本10例作对照.采用第二代通用型二步法监测系统(PV-6000)免疫组织化学方法检测RECK及MMP-14蛋白的表达.结果 RECK蛋白在神经母细胞瘤组织中低表达(16.7%),并随肿瘤浸润深度的加深、远处转移的发生而降低(P=0.015<0.05).MMP-14在神经母细胞瘤组织中高表达(58.3%),并随肿瘤浸润深度的加深、远处转移的发生而增高(P=0.002<0.05).神经母细胞瘤组织中RECK与MMP-14蛋白的表达呈负相关(r=-0.418,P<0.05).结论神经母细胞瘤组织中RECK低表达,而MMP-14高表达.RECK及MMP-14可能成为评估神经母细胞瘤发生浸润转移的重要指标.
목적 검측RECK단백급막형기질금속단백매MMP-14재신경모세포류중적표체,탐토기재신경모세포류적발생、발전、침윤화전이중적작용.방법 선택림상급병리자료제전적존당신경모세포류사괴표본36례,령취절세포신경류사괴표본10례작대조.채용제이대통용형이보법감측계통(PV-6000)면역조직화학방법 검측RECK급MMP-14단백적표체.결과 RECK단백재신경모세포류조직중저표체(16.7%),병수종류침윤심도적가심、원처전이적발생이강저(P=0.015<0.05).MMP-14재신경모세포류조직중고표체(58.3%),병수종류침윤심도적가심、원처전이적발생이증고(P=0.002<0.05).신경모세포류조직중RECK여MMP-14단백적표체정부상관(r=-0.418,P<0.05).결론 신경모세포류조직중RECK저표체,이MMP-14고표체.RECK급MMP-14가능성위평고신경모세포류발생침윤전이적중요지표.
Objective To detect the expression of RECK (reversion-inducing eysteine-rieh protein with Kazal motifs), MMP-14 (matrix metalloproteinase-14), as well as their correlations in neuroblastoma and to investigate their roles in metastasis and development of the tumor. Methods PV-6000 immunohisto-chemistry was used to measure the expression of RECK and MMP-14, in 36 samples of neuroblastoma tissue selected from paraffin wax-embodied specimens with complete clinicopathological data,and another 10 cases of ganglioneuroma tissue. Results The expression rate of RECK in neuroblastoma was low (16.7%), and re-duced with the increased invasive depth and long-distance metastasis(P=0.015<0.05). The expression rate of MMP-14 in neuroblastoma was high (58.3%),and elevated with the increased invasive depth and long-distance metastasis (P=0.002<0.05). The expression of RECK was negatively correlated with the expres-sion of MMP-14 (r=-0.418, P<0.05). Conclusions RECK is lowly expressed in neuroblastoma, but MMP-14 is highly expressed. RECK,MMP-14 may be served as the markers in the estimation of metastasis and dissemination of neuroblastoma.