中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2009年
10期
861-866
,共6页
张顺祥%谷利妞%何建凡%程小雯%胡东生%吕星%吴春利%逯建华%房师松
張順祥%穀利妞%何建凡%程小雯%鬍東生%呂星%吳春利%逯建華%房師鬆
장순상%곡리뉴%하건범%정소문%호동생%려성%오춘리%록건화%방사송
流感病毒A型%H3N2亚型%序列分析%人群监测%流行病学研究
流感病毒A型%H3N2亞型%序列分析%人群鑑測%流行病學研究
류감병독A형%H3N2아형%서렬분석%인군감측%류행병학연구
Influenza A virus%H3N2 subtype%Sequence analysis%Population surveillance%Epidemiologic studies
目的 探讨深圳市2005-2007年季节性流行性感冒(简称流感)流行病学特征和甲3亚型流感病毒可能的分子变异.方法 对深圳市2005-2007年流感监测网络(由9所医院、6个区和市疾病预防控制中心共16个主要机构组成)的运行质量进行控制,按周统计分析主要监测医院流感样病例占就诊者的百分比(ILI百分比),并采集流感样病例鼻咽拭子进行流感病毒分离鉴定,对分离到的毒株提取病毒RNA,用反转录聚合酶链式反应(RT-PCR)扩增血凝素基因重链区(HAI区)基因片段,产物纯化后测序并进行核苷酸相似性、基因进化树和氨基酸变异分析.结果 深圳市2005-2007年流感活动水平在各年间有所波动,呈现夏季高峰,2006和2007年5-7月为流行高峰期.2005-2007年流感病毒的分离率分别为4.78%(114/2385)、5.77%(212/3674)和12.12%(343/2831),分离毒株数多少与ILI百分比的季节波动相关,按周分离率与ILI百分比的变化趋势基本一致.2005和2006年甲3亚型所占比例分别为25.46%(28/114)和2.83%(6/212),而2007年成为优势毒株,占62.68%(215/343).甲3亚型病毒HA1区序列进化树分析发现,虽然进化树的主干基本按照毒株分离的时间先后发展,与不同年度WHO推荐的疫苗株先后一致,但2005-2007年的主要流感毒株共分为5个分支,2005-2006年毒株在第Ⅰ～Ⅲ分支,2007年4-6月和5-12月形成2个分支,与同期WHO推荐的疫苗株不在一个进化分支,深圳甲3亚型流感病毒的变化较早,出现疫苗株滞后现象.所有毒株未见HA1区序列缺失和插入,受体结合位点和二硫键位点氨基酸也显保守,一些位点出现了不同属性氨基酸的置换,并导致个别潜在的糖基化位点的丢失或增加,但尚不能判定为新变种.结论 深圳市可能属于我国流感病毒变异较早的地区之一,甲3亚型流感病毒在人群中呈活跃状态;常规季节性流感监测与病毒分子变异分析结合,能为毒株的演变及其流行病学意义提供及时、重要的信息.
目的 探討深圳市2005-2007年季節性流行性感冒(簡稱流感)流行病學特徵和甲3亞型流感病毒可能的分子變異.方法 對深圳市2005-2007年流感鑑測網絡(由9所醫院、6箇區和市疾病預防控製中心共16箇主要機構組成)的運行質量進行控製,按週統計分析主要鑑測醫院流感樣病例佔就診者的百分比(ILI百分比),併採集流感樣病例鼻嚥拭子進行流感病毒分離鑒定,對分離到的毒株提取病毒RNA,用反轉錄聚閤酶鏈式反應(RT-PCR)擴增血凝素基因重鏈區(HAI區)基因片段,產物純化後測序併進行覈苷痠相似性、基因進化樹和氨基痠變異分析.結果 深圳市2005-2007年流感活動水平在各年間有所波動,呈現夏季高峰,2006和2007年5-7月為流行高峰期.2005-2007年流感病毒的分離率分彆為4.78%(114/2385)、5.77%(212/3674)和12.12%(343/2831),分離毒株數多少與ILI百分比的季節波動相關,按週分離率與ILI百分比的變化趨勢基本一緻.2005和2006年甲3亞型所佔比例分彆為25.46%(28/114)和2.83%(6/212),而2007年成為優勢毒株,佔62.68%(215/343).甲3亞型病毒HA1區序列進化樹分析髮現,雖然進化樹的主榦基本按照毒株分離的時間先後髮展,與不同年度WHO推薦的疫苗株先後一緻,但2005-2007年的主要流感毒株共分為5箇分支,2005-2006年毒株在第Ⅰ～Ⅲ分支,2007年4-6月和5-12月形成2箇分支,與同期WHO推薦的疫苗株不在一箇進化分支,深圳甲3亞型流感病毒的變化較早,齣現疫苗株滯後現象.所有毒株未見HA1區序列缺失和插入,受體結閤位點和二硫鍵位點氨基痠也顯保守,一些位點齣現瞭不同屬性氨基痠的置換,併導緻箇彆潛在的糖基化位點的丟失或增加,但尚不能判定為新變種.結論 深圳市可能屬于我國流感病毒變異較早的地區之一,甲3亞型流感病毒在人群中呈活躍狀態;常規季節性流感鑑測與病毒分子變異分析結閤,能為毒株的縯變及其流行病學意義提供及時、重要的信息.
목적 탐토심수시2005-2007년계절성류행성감모(간칭류감)류행병학특정화갑3아형류감병독가능적분자변이.방법 대심수시2005-2007년류감감측망락(유9소의원、6개구화시질병예방공제중심공16개주요궤구조성)적운행질량진행공제,안주통계분석주요감측의원류감양병례점취진자적백분비(ILI백분비),병채집류감양병례비인식자진행류감병독분리감정,대분리도적독주제취병독RNA,용반전록취합매련식반응(RT-PCR)확증혈응소기인중련구(HAI구)기인편단,산물순화후측서병진행핵감산상사성、기인진화수화안기산변이분석.결과 심수시2005-2007년류감활동수평재각년간유소파동,정현하계고봉,2006화2007년5-7월위류행고봉기.2005-2007년류감병독적분리솔분별위4.78%(114/2385)、5.77%(212/3674)화12.12%(343/2831),분리독주수다소여ILI백분비적계절파동상관,안주분리솔여ILI백분비적변화추세기본일치.2005화2006년갑3아형소점비례분별위25.46%(28/114)화2.83%(6/212),이2007년성위우세독주,점62.68%(215/343).갑3아형병독HA1구서렬진화수분석발현,수연진화수적주간기본안조독주분리적시간선후발전,여불동년도WHO추천적역묘주선후일치,단2005-2007년적주요류감독주공분위5개분지,2005-2006년독주재제Ⅰ～Ⅲ분지,2007년4-6월화5-12월형성2개분지,여동기WHO추천적역묘주불재일개진화분지,심수갑3아형류감병독적변화교조,출현역묘주체후현상.소유독주미견HA1구서렬결실화삽입,수체결합위점화이류건위점안기산야현보수,일사위점출현료불동속성안기산적치환,병도치개별잠재적당기화위점적주실혹증가,단상불능판정위신변충.결론 심수시가능속우아국류감병독변이교조적지구지일,갑3아형류감병독재인군중정활약상태;상규계절성류감감측여병독분자변이분석결합,능위독주적연변급기류행병학의의제공급시、중요적신식.
Objective To determine the epideroiological characteristics of seasonal influenza in Shenzhen from 2005 to 2007 and the molecular variation of HA1 domain of influenza H3N2 viruses. Methods The consultation rate for influenza-like illness (ILI) were calculated weekly for indicating the influenza activities(the Shenzhen Influenza Surveillance System mainly consisted of 16 institutions with 9 hospitals,6 districts and one municipal centers of disease control and prevention). Pharyngeal swabs from the cases of ILI, which were collected during 2005 to 2007 from the city-wide and quality-controlled surveillance network, were used to propagate the viruses. The HA1 region of the influenza A/H3N2 viruses were detected by RT-PCR and sequenced subsequently. The analyses of pairwise amino acid variations, genetic clustering and phylogeneties was performed. Results The activity levels of influenza showed certain changes during each year from 2005 to 2007,and there were summer peaks from May to July in 2006 and 2007. The positive rates of influenza virus were 4.78% (114/2385),5.77% (212/3674) and 12.12% (343/2831) from 2005 to 2007 respectively. The weekly isolating rates changed accordantly with the trend of the percentages of ILI. The proportions of influenza H3N2 virus were 25.46% (28/114) and 2. 83% (6/212) in 2005 and in 2006 respectively,but the proportion increased to 62. 68% (215/343), which indicated that H3N2 virus became the predominant strain in 2007. Phylogenetic clustering analysis of influenza H3 N2 virus revealed that there were 5 elades. The viruses which were isolated in 2005 contained in the clade Ⅰ and Ⅱ,the viruses in 2006 were comprised in clade Ⅲ, and clade Ⅳ and Ⅴ included the viruses isolated in 2007. Although the stem of cladogram developed with one accord of the time isolated viruses, the viruses which were similar to vaccine strains had circulated in Shenzhen before a given strain was determined as vaccine strain by WHO. It was also noticed that more amino acid changes at antigenic sites,especially at sites A and B in the H3N2 viruses,were found in 2007 than that in 2005 and in 2006. But the sequences at the receptor-binding sites and disulphide bond sites were conserved and no new circulating strain for genetic reassortment had been found in the period. Conclusion Shenzhen might be one of areas where the ongoing genetic drift of influenza H3N2 viruses appeared earlier in China. The changes of influenza H3N2 virus showed the active status in the population. The results suggested that monitoring seasonal influenza viruses by sequence analysis could provide important and timely information on the appearance of strains with epidemiologic significance.
