CAJ | 학술논문

目的了解中国朝鲜族群体线粒体DNA(mitochondrial DNA,mtDNA)编码区3954-4506nt的序列多态性.方法采用PCR产物直接测序方法对198名吉林省延边朝鲜族自治州朝鲜族无关个体进行单倍型分布调查.结果在198名无关个体中,共分出21种单倍型.遗传变异度为0.5906,偶合概率为0.4124.测序结果与Anderson序列比较,共检测出19个变异位点,14个与MITOMAP收录的基因突变相同,5个未见收录.结论 mtDNA编码区多态性位点作为mtDNA控制区多态性位点的补充,联合应用可以提高mtDNA的个体识别能力,为mtDNA在中国朝鲜族法医学鉴定提供了基础数据.
목적 료해중국조선족군체선립체DNA(mitochondrial DNA,mtDNA)편마구3954-4506nt적서렬다태성.방법 채용PCR산물직접측서방법 대198명길림성연변조선족자치주조선족무관개체진행단배형분포조사.결과 재198명무관개체중,공분출21충단배형.유전변이도위0.5906,우합개솔위0.4124.측서결과 여Anderson서렬비교,공검측출19개변이위점,14개여MITOMAP수록적기인돌변상동,5개미견수록.결론 mtDNA편마구다태성위점작위mtDNA공제구다태성위점적보충,연합응용가이제고mtDNA적개체식별능력,위mtDNA재중국조선족법의학감정제공료기출수거.
Objective To investigate the sequence polymorphism of mtDNA coding region encompassing nt3954-4506 in the Korean Chinese population of Yanbian area.Methods Polymerase chain reaction(PCR)and direct sequencing method were used to detect the haplotype distribution of mtDNA coding region in 198 Korean Chinese individuals.Results Twenty-one haplotypes were observed in the 198 unrelated individuals.The genetic diversity was 0.5906 and the random match probability was 0.4124.Compared with the Anderson's sequence.19 nucleotide variants were found,of which 14 were previously registered in MITOMAP,and 5 were novel.Conclusion The obtained data suggest that these sequence polymorphisms are valuable genetic markers for personal identification when combined with mtDAN control region investigation,thus could be used as basic data for the forensic application in Korean Chinese population.