CAJ | 학술논문

嗜麦芽寡养单胞菌可共代谢降解烟碱类农药吡虫啉,并且对多种抗生素具有抗性.本文通过电穿孔法消除了该菌的四环素抗性,获得了四环素敏感型菌株R551-3~(Tcr-),可以用作遗传转化系统的受体菌,能被转化吸收遗传载体质粒pJB866H::ndhSL,构成一对很好的嗜麦芽寡养单胞菌遗传转化系统.同时HPLC结果表明所获得的四环素敏感型菌株羟基化吡虫啉的活性并未发生改变,为进一步开展敲除和回补共代谢途径关键酶的研究工作奠定了基础.
기맥아과양단포균가공대사강해연감류농약필충람,병차대다충항생소구유항성.본문통과전천공법소제료해균적사배소항성,획득료사배소민감형균주R551-3~(Tcr-),가이용작유전전화계통적수체균,능피전화흡수유전재체질립pJB866H::ndhSL,구성일대흔호적기맥아과양단포균유전전화계통.동시HPLC결과표명소획득적사배소민감형균주간기화필충람적활성병미발생개변,위진일보개전고제화회보공대사도경관건매적연구공작전정료기출.
Stenotrophomonas maltophilia can degrade neonicotinoid insecticide imidacloprid through co-metabolism and exhibits multiple antibiotic resistance. Through electroporation we obtained a R551-3 mutant which lost tetracycline resistance. Besides, the tetracycline resistant plasmid pJB866H::ndhSL was successfully transformed into the R551-3~(Tcr-) strain. Therefore, the R551-3~(Tcr-) strain can be used as a recipient to construct a Stenotrophomonas maltophilia genetic transformation system. The tetracycline resistance lost mutant keeps the ability of hydroxylating imidacloprid. Based on this mutant, the following gene knockout and recovery experiments can be carried out.