标记辅助培育水稻抗稻褐飞虱和稻白叶枯病基因聚合系
표기보조배육수도항도갈비슬화도백협고병기인취합계
Rice Pyramiding Lines Conferring Resistances to Brown Planthopper and Bacterial Leaf Blight by Marker-Assisted Selection
  • 万方数据
    分子植物育种 분자식물육충
    MOLECULAR PLANT BREEDING
    2010年 1期 11-19 ,共9页

    阳海宁%韦绍丽%李孝琼%张月雄%刘驰%陈乔%岑贞陆%韦素美%杨朗%黄凤宽%何文强%刘开雨%黄大辉%马增凤%黄悦悦%宋建东%李容柏

    양해저%위소려%리효경%장월웅%류치%진교%잠정륙%위소미%양랑%황봉관%하문강%류개우%황대휘%마증봉%황열열%송건동%리용백

    杂交水稻%保持系%Bph3Xa23基因聚合%基因导入系%设计育种 雜交水稻%保持繫%Bph3Xa23基因聚閤%基因導入繫%設計育種
    잡교수도%보지계%Bph3Xa23기인취합%기인도입계%설계육충
    Hybrid rice%Maintainer%Bph3Xa23 pyramiding%Gene-insered line%Design breeding
    稻白叶枯病和稻褐飞虱是水稻的主要病虫害,利用优良的抗性基因培育抗性基因聚合系对于防治稻白叶枯病和稻褐飞虱具有重要意义.本研究通过回交与分子标记辅助选择相结合的方法,将抗稻白叶枯病基因Xa23和抗稻褐飞虱基因Bph3分别导入主栽杂交水稻品种的保持系先B、天B、盟B、龙特甫B和桂B中.结果表明,获得稳定的单抗性基因导入系1 436份和双抗性基因聚合系144份.人工接种鉴定结果显示,Xa23导入系和Xa23Bph3聚合系对抗稻白叶枯水平达到中抗和接近高抗的水平(1.1～3.0级),Bph3导入系和Xa23Bph3聚合系抗褐飞虱水平达中抗到抗的级别(3.2～4.0级).农艺性状分析显示多数抗性基因聚合系的株高、剑叶长宽度、每穗粒数、结实率、千粒重等主要农艺性状与受体差异不显著,只有少数聚合系有1～2个性状的差异.SSR标记分析表明抗性基因聚合系的遗传背景回复率达89.6%97.8%,等位性位点纯合度达95.6%～99.9%.初步证明本研究已成功地获得抗稻白叶枯病和稻褐飞虱基因的聚合系,这些抗性改良保持系具有良好的应用前景,并为建立完善的分子设计育种平台提供了重要育种材料基础.
    도백협고병화도갈비슬시수도적주요병충해,이용우량적항성기인배육항성기인취합계대우방치도백협고병화도갈비슬구유중요의의.본연구통과회교여분자표기보조선택상결합적방법,장항도백협고병기인Xa23화항도갈비슬기인Bph3분별도입주재잡교수도품충적보지계선B、천B、맹B、룡특보B화계B중.결과표명,획득은정적단항성기인도입계1 436빈화쌍항성기인취합계144빈.인공접충감정결과현시,Xa23도입계화Xa23Bph3취합계대항도백협고수평체도중항화접근고항적수평(1.1～3.0급),Bph3도입계화Xa23Bph3취합계항갈비슬수평체중항도항적급별(3.2～4.0급).농예성상분석현시다수항성기인취합계적주고、검협장관도、매수립수、결실솔、천립중등주요농예성상여수체차이불현저,지유소수취합계유1～2개성상적차이.SSR표기분석표명항성기인취합계적유전배경회복솔체89.6%97.8%,등위성위점순합도체95.6%～99.9%.초보증명본연구이성공지획득항도백협고병화도갈비슬기인적취합계,저사항성개량보지계구유량호적응용전경,병위건립완선적분자설계육충평태제공료중요육충재료기출.
    Plant brownhopper (BPH,caused by Nilaparvata lugens (St(a)l)) and bacterial leaf blight (BLB,caused by Xanthomonas campestris PV.Oryzae) are two of the major diseases in rice.To breed pyramided lines of the two disease-resistance genes are of great importance for the control of the two diseases.In this research,BLB and BPH resistance genes,Xa23 and Bph3,were accumulated into 5 maintainers of current main cultivated riee hybrids of Guangxi,Xian B,Tianfeng B,Meng B,Longtepu B and Gui B,via backerossing together with molecular markerassisted selection (MAS).As a result,we obtained 1436 single resistance gene-inserted lines and 144 double resistance gene-accumulated lines.Data from the inoculation with the BLB pathogen and BPH insect showed that the Xa23-inserted lines and Xa23Bph3 accumulated lines were moderately resistant or near highly resistant to the BLB with resistance scores 1.1～3.0,while the Bph3-inserted lines and Xa23Bph3 accumulated lines were moderately resistant to the BPH with resistance scores 3.2～4.0.Agronomic traits analysis reveal that there were no significant differences between most of the gene-accumulated lines and receptor parents in plant height,flag leaf length and breadth,grain number per panicle,filled grained rate and 1 000-grain weight,except one or two traits in a few accumulated lines.SSR analysis showed that the recovery rate of the resistance gene-accumulated lines was up to 89.6%～97.8% of the receptors,and their allelic homozygosity reached 95.6%～99.9%.The above results preliminary demonstrated the BPH and BLB resistance accumulated lines were successfully bred and these resistance rice maintainers were quite potential in developing new promising resistant hybrid varieties.This work would also provide a solid foundation in breeding material for the establishment of the molecular design breeding platform.
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