CAJ | 학술논문

本研究利用来自Cotton Marker Database(CMD)数据库中的2 000个引物对32份审定棉花品种基因组DNA进行了PCR扩增,筛选出26个多态性核心引物,其中11个引物被推荐为棉花品种鉴定的首选核心引物,同时提出了核心引物理论上可区分的最大品种数的计算公式:N=G1x…xGn,理论推测结果表明:利用11个首选核心引物进行棉花品种鉴定是可行的.建立在SSR指纹数据基础上的不同组合品种(系)间聚类分析以及对25份区试品系和A品种的真实性鉴定,结果表明:所有参试品种(系)遗传基础狭窄,32份审定品种间DNA水平上的差异与品种来源的系谱分析结果基本吻合,首选核心引物不仅可以对品种(系)的真实性做出鉴定,而且对遗传距离狭窄的品种(系)具有很好的鉴别力.
본연구이용래자Cotton Marker Database(CMD)수거고중적2 000개인물대32빈심정면화품충기인조DNA진행료PCR확증,사선출26개다태성핵심인물,기중11개인물피추천위면화품충감정적수선핵심인물,동시제출료핵심인물이론상가구분적최대품충수적계산공식:N=G1x…xGn,이론추측결과표명:이용11개수선핵심인물진행면화품충감정시가행적.건립재SSR지문수거기출상적불동조합품충(계)간취류분석이급대25빈구시품계화A품충적진실성감정,결과표명:소유삼시품충(계)유전기출협착,32빈심정품충간DNA수평상적차이여품충래원적계보분석결과기본문합,수선핵심인물불부가이대품충(계)적진실성주출감정,이차대유전거리협착적품충(계)구유흔호적감별력.
In this study, genomic DNA from thirty-two approved cotton cultivars were amplified with 2 000 primer pairs selecting from cotton marker database (CMD) by using polymerase chain reaction (PCR), twenty-six core primer pairs with polymorphism were selected, of which eleven core primer pairs were proposed as preferred primer pairs for identification of cotton cultivars, at the same time a formula for calculating the maximum number of cotton cultivars that the eleven core primer pairs can identify in theory was advanced: N=G1x...xGn, and according to theory, it has deduced that eleven core primer pairs were effective. Genetic clustering analysis of different combinations of cotton cutlivars (lines) used in this study and authenticity identification of A approved cutlivar and twenty-five cotton varieties in Shandong provincial regional trial were performed based on SSRs fingerprint database, the result showed that genetic basis of the tested materials were limited, difference of thirty-two approved cotton cultivars at DNA level was in accordance with the result of pedigree analysis of them, the preferred eleven core prime pairs not only can identify the authenticity of cotton cultivars (lines), but also had good power in identification of cotton cultivars (lines) with limited genetic basis.