CAJ | 학술논문

目的:研究紫草素对DNA拓扑异构酶I催化活性和K562白血病细胞凋亡的影响.方法:从K562白血病细胞提取拓扑异构酶I,通过DNA解螺旋试验评价该药对拓扑异构酶I催化活性的抑制作用.用MTT法测定了该药对K562白血病细胞增殖的抑制作用.应用流式细胞术和琼脂糖凝胶电泳观察了该药对细胞凋亡的诱导作用.结果:紫草素可显著抑制拓扑异构酶I的解螺旋活性(IC50=75.0 μmol/L).该药能显著抑制K562的细胞增殖,并随剂量增大而抑制作用增强.紫草素0.3～3 μmol/L对K562细胞作用24 h后,其凋亡率为20%～70%.琼脂糖凝胶电泳出现典型的DNA"ladder".结论:紫草素显著抑制拓扑异构酶I的催化活性,并能诱导K562白血病细胞凋亡.
목적:연구자초소대DNA탁복이구매I최화활성화K562백혈병세포조망적영향.방법:종K562백혈병세포제취탁복이구매I,통과DNA해라선시험평개해약대탁복이구매I최화활성적억제작용.용MTT법측정료해약대K562백혈병세포증식적억제작용.응용류식세포술화경지당응효전영관찰료해약대세포조망적유도작용.결과:자초소가현저억제탁복이구매I적해라선활성(IC50=75.0 μmol/L).해약능현저억제K562적세포증식,병수제량증대이억제작용증강.자초소0.3～3 μmol/L대K562세포작용24 h후,기조망솔위20%～70%.경지당응효전영출현전형적DNA"ladder".결론:자초소현저억제탁복이구매I적최화활성,병능유도K562백혈병세포조망.
AIM:To study the effects of shikonin on the catalytic activity of DNA topoisomerase I and apoptosis of K562 leukemia cells. METHOD: Topoisomerase I was extracted from K562 cells and the inhibitory effect of shikonin on the catalytic activity was estimated by DNA relaxation assay. MTT assay was used to measure the inhibition of K562 cells proliferation. The amount of apoptotics cells was determined by flow cytometry (FCM). DNA fragmentation was analyzed by agarose gel electrophoresis. RESULT:Shikonin remarkadly inhibited the catalytic activity of topoisomerase I with IC50 value of 75.0 μmol/L. K562 cells proliferation was inhibited by shikonin in a concentration-dependent manner. The analysis with flow cytometry showed that the percentage of apoptotic cells was about 20%～70% after K562 cells were treated by 0.3～3 μmol/L shikonin for 24 h, and DNA ladder can be observed by electrophoretic analysis. CONCLUSION: This study showed that shikonin might inhibit the catalytic activity of DNA topoisomerase I and have apoptosis-induced activity for K562 leukemia cells.