CAJ | 학술논문

背景:课题组自主研发一种仿松质骨生物活性纳米人工骨,在临床试验前进行系列的动物实验提供必需的技术资料.目的:评价自主研发的仿松质骨胶原/纳米羟基磷灰石人工骨的生物可降解性、骨引导性和骨诱导性.设计、时间及地点:随机对照动物实验,于2007-03-01/06-08在广东省医学实验动物中心完成.材料:纳米羟基磷灰石粉末通过共沉淀反应合成.将纳米羟基磷灰石粉末按一定比例加入胶原溶液中充分混合,然后冷冻干燥即得块状纳米人工骨.15只新西兰大白兔,随机分成3组,每组5只,分别为空白对照组、羟基磷灰石珊瑚组和纳米人工骨组.方法:所有动物局部麻醉后在一侧尺骨造成10 mm全缺损,纳米人工骨组和羟基磷灰石珊瑚组分别植入纳米人工骨、羟基磷灰石珊瑚,空白对照组不植入任何物质.在植入后30,60 d,通过大体观察、X射线摄片、电子显微镜及组织学评价该人工骨的的生物相容性和骨诱导性.主要观察指标:纳米人工骨的超微结构,创面愈合情况,人工骨降解情况和缺损区骨组织再生情况.结果:该人工骨具有与天然松质骨相似的内连孔结构、孔隙率和孔径,这种结构有利于骨细胞的长入和血管新生;植入后30 d可见纳米人工骨组缺损处破纳米人工骨修复,人工骨被彻底降解.植入后60d羟基磷灰石珊瑚组未见骨修复和骨降解,仅见大量软组织再生.结论:自制仿松骨胶原/纳米羟基磷灰石人工骨可降解,成骨效果好,可替代自体骨移植修复大段骨缺损.
배경:과제조자주연발일충방송질골생물활성납미인공골,재림상시험전진행계렬적동물실험제공필수적기술자료.목적:평개자주연발적방송질골효원/납미간기린회석인공골적생물가강해성、골인도성화골유도성.설계、시간급지점:수궤대조동물실험,우2007-03-01/06-08재광동성의학실험동물중심완성.재료:납미간기린회석분말통과공침정반응합성.장납미간기린회석분말안일정비례가입효원용액중충분혼합,연후냉동간조즉득괴상납미인공골.15지신서란대백토,수궤분성3조,매조5지,분별위공백대조조、간기린회석산호조화납미인공골조.방법:소유동물국부마취후재일측척골조성10 mm전결손,납미인공골조화간기린회석산호조분별식입납미인공골、간기린회석산호,공백대조조불식입임하물질.재식입후30,60 d,통과대체관찰、X사선섭편、전자현미경급조직학평개해인공골적적생물상용성화골유도성.주요관찰지표:납미인공골적초미결구,창면유합정황,인공골강해정황화결손구골조직재생정황.결과:해인공골구유여천연송질골상사적내련공결구、공극솔화공경,저충결구유리우골세포적장입화혈관신생;식입후30 d가견납미인공골조결손처파납미인공골수복,인공골피철저강해.식입후60d간기린회석산호조미견골수복화골강해,부견대량연조직재생.결론:자제방송골효원/납미간기린회석인공골가강해,성골효과호,가체대자체골이식수복대단골결손.
BACKGROUND: A nano-artificial bone imitating cancellous bone has been developed. It is necessary to perform a series of animal experiments regarding this artificial bone prior to clinical trials for providing technical information.OBJECTIVE: To evaluate the biodegradability, osteoconductivity, and osteoinductivity of nano-hydroxyapatite/collagen (nano-HA/CO) composites imitating cancellous bone.DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at the Guangdong Medical Laboratory Animal Center between March and June 2007.MATERIALS: The HA powder was synthesized by a co-precipitation reaction. The obtained HA powder was added into collagen solution at a certain ratio under vigorous stirring. The nano-HA/CO composites were frozen-dried to obtain nano-artificial bone. Fifteen New Zealand rabbits were randomly divided into 3 groups, with 5 rats per group: blank control, coral-HA, and nano-HA/CO.METHODS: Following local anesthesia, 10 mm bone defect was made on the right ulnas in all rabbits. HA coral and Nano-HA/CO bone composites were inserted into the defects in the HA and nano-HA/CO groups, respectively. The blank control group received no any implantation. At 30 and 60 days following surgery, biocompatibility and osteoinductivity of cancellous bone resembling nano-HA/CO bone substitute were assessed by macroscopic examination, scanning electron microscopy, X-ray photography, and histological examination after implantation in vivo.MAIN OUTCOME MEASURES: Ultrastructure and degradation condition of nano-HA/CO bone substitute, postoperative wound healing, and osteo-regeneration in defect region.RESULTS: The nano-HA/CO bone composite possessed interconnected porosity and pore size resembling cancellous bone, which benefits in-growth of osteoblasts and blood vessels. At 30 days following surgery, the nano-HA/CO group exhibited that osteoblasts spread around, and ossification occurred in almost all newly formed bone area. At 60 days following surgery, the coral-HA group showed that only connective tissue regenerated without bone tissue regeneration and degradation of grafts.CONCLUSION: This nano-HA/CO bone composite shows great promise in repairing massive bone defect in stead of autograft.