CAJ | 학술논문

基于分离理论的共性,通过薄层层析优化难分离物质对的最佳分离条件并直接放大到植物天然产物的工业制备分离.本文对实际应用中的溶剂组成、溶剂强度、溶剂用量及溶剂再利用等问题作了详细论述.最佳溶剂选择性可通过不同溶剂系统在薄层板上所给难分离物质对的最大Rf比值确定;在正相硅胶层析中,主要极性溶剂决定了物质的洗脱顺序;洗脱剂中极少量强极性溶剂(0.5%)或pH值的改变可显著改善拖尾现象;根据Rf值和容量因子(K)及洗脱体积三者之间的关系,Rf在0.1～0.3为用于柱层析的洗脱溶剂强度的最佳范围.
기우분리이론적공성,통과박층층석우화난분리물질대적최가분리조건병직접방대도식물천연산물적공업제비분리.본문대실제응용중적용제조성、용제강도、용제용량급용제재이용등문제작료상세논술.최가용제선택성가통과불동용제계통재박층판상소급난분리물질대적최대Rf비치학정;재정상규효층석중,주요겁성용제결정료물질적세탈순서;세탈제중겁소량강겁성용제(0.5%)혹pH치적개변가현저개선타미현상;근거Rf치화용량인자(K)급세탈체적삼자지간적관계,Rf재0.1～0.3위용우주층석적세탈용제강도적최가범위.
On the basis of the commonness theory of separation, optimum isolation conditions for components diffi-cult to be separated are established by using thin layer chromatography (TLC), and directly scale up to industrialpreparative chromatography (IPC) of plant-derived natural products. Solvent composition, solvent strength,solventdosage and solvent re-utilization in the practical application are evaluated. The best solvent selectivity for IPC can beobtained by comparing the ratio of Rf value of different solvent systems on TLC plate with compounds being isolated.The dominant polar solvent in eluent govern the peak sequence,and a little of more polar solvent or pH change canmodify tailing peak. And according to the relationship between Rf and capacity factor (K) and eluting volumes, thebest range of solvent strength for IPC judged with Rf value is confirmed from 0.1 to 0.3.