CAJ | 학술논문

目的　探讨T细胞接种(TCV)对1型糖尿病的预防作用。方法　将6周龄未发病的雌性NOD鼠用4周龄未发病、18周龄新近发病和32周龄发病时间长的NOD鼠脾细胞制得的T细胞疫苗接种，检测TCV对环磷酰胺处理的NOD鼠糖尿病发病率和胰岛炎程度的影响，以及接种后宿主淋巴细胞亚群的变化情况。结果　TCV可以降低糖尿病发病率、减轻胰岛单个核细胞的浸润程度，诱导宿主脾脏CD8+ T细胞百分比升高、CD4+/CD8+比值和IL-2R+细胞下降，胸腺CD4- CD8+单阳性细胞百分比升高。结论　TCV可以降低宿主对自身免疫的反应性，这种作用可能与宿主脾脏、胸腺淋巴细胞亚群的变化和对糖尿病的预防效应有关。
목적　탐토T세포접충(TCV)대1형당뇨병적예방작용。방법　장6주령미발병적자성NOD서용4주령미발병、18주령신근발병화32주령발병시간장적NOD서비세포제득적T세포역묘접충，검측TCV대배린선알처리적NOD서당뇨병발병솔화이도염정도적영향，이급접충후숙주림파세포아군적변화정황。결과　TCV가이강저당뇨병발병솔、감경이도단개핵세포적침윤정도，유도숙주비장CD8+ T세포백분비승고、CD4+/CD8+비치화IL-2R+세포하강，흉선CD4- CD8+단양성세포백분비승고。결론　TCV가이강저숙주대자신면역적반응성，저충작용가능여숙주비장、흉선림파세포아군적변화화대당뇨병적예방효응유관。
Objective　To explore the prophylactic effect of T cell vaccination (TCV) on type 1 diabetes. Methods　6-week-old nondiabetic femele NOD mice were vaccinated intraperitoneally with the attenuated, activated T lymphocytes isolated from the spleen of 4-week-old nondiabetic NOD mice, 18-week-old newly diabetic NOD mice and 32-week-old long-term diabetic NOD mice. The incidence of cyclophosphamide-treated diabetes, inflammatory score of insulitis in NOD mice as well as T lymphocyte subset changes in spleen and thymus were determined after TCV. Results　TCV was able to reduce the incidence of cyclophosphamide-treated diabetes, to alleviate insulitis, to increase the percentage of CD8+ T lymphocyte subset in spleen and CD4- CD8+ simple-positive T lymphocyte in thymus, and to decrease the percentage of IL-2R+ T lymphocyte and CD4+/CD8+ rate in spleen. Conclusion　TCV may decrease host autoimmunity, which seems related to the changes of T lymphocyte subsets in spleen and thymus and prophylactic effect on diabetes.