植物分类学报
植物分類學報
식물분류학보
ACTA PHYTOTAXONOMICA SINICA
2008年
2期
194-204
,共11页
沈锦波%吕洪飞%彭秋发%郑菊芳%田玉梅
瀋錦波%呂洪飛%彭鞦髮%鄭菊芳%田玉梅
침금파%려홍비%팽추발%정국방%전옥매
山茶属%聚类分析%红外光谱%分类学意义
山茶屬%聚類分析%紅外光譜%分類學意義
산다속%취류분석%홍외광보%분류학의의
Camellia%cluster analysis%infrared spectra%taxonomic significance
利用傅立叶红外光谱仪和OMNI采样器直接、迅速、准确地测定山茶属Camellia4组63种2变种植物叶片的红外光谱,结果表明:各分类群(种)的红外光谱具有高度特异性,其红外光谱图的变化可以作为山茶属植物属下的分类依据之一.这也暗示了利用标准红外光谱图库,可以区分和鉴定出山茶属植物的种类.经主成分分析后的红外光谱数据构建的树型聚类图与先前的形态分类结果大体一致,能将油茶组sect.Oleifera和短柱茶组sect.Paracamellia植物明显区分,并且各组中亲缘关系较近的种聚在一起.因此支持它们作为两个独立的组处理.但是,红山茶组sect.Camellia内的滇山茶亚组subsect.Lucidissima和光果红山茶亚组subsect.Reticulata植物在聚类图上很难区分,建议将这两个亚组植物进行归并.最后讨论了张宏达和闵天禄系统中存在分歧的油茶组、短柱茶组和红山茶组内的种间分类关系.
利用傅立葉紅外光譜儀和OMNI採樣器直接、迅速、準確地測定山茶屬Camellia4組63種2變種植物葉片的紅外光譜,結果錶明:各分類群(種)的紅外光譜具有高度特異性,其紅外光譜圖的變化可以作為山茶屬植物屬下的分類依據之一.這也暗示瞭利用標準紅外光譜圖庫,可以區分和鑒定齣山茶屬植物的種類.經主成分分析後的紅外光譜數據構建的樹型聚類圖與先前的形態分類結果大體一緻,能將油茶組sect.Oleifera和短柱茶組sect.Paracamellia植物明顯區分,併且各組中親緣關繫較近的種聚在一起.因此支持它們作為兩箇獨立的組處理.但是,紅山茶組sect.Camellia內的滇山茶亞組subsect.Lucidissima和光果紅山茶亞組subsect.Reticulata植物在聚類圖上很難區分,建議將這兩箇亞組植物進行歸併.最後討論瞭張宏達和閔天祿繫統中存在分歧的油茶組、短柱茶組和紅山茶組內的種間分類關繫.
이용부립협홍외광보의화OMNI채양기직접、신속、준학지측정산다속Camellia4조63충2변충식물협편적홍외광보,결과표명:각분류군(충)적홍외광보구유고도특이성,기홍외광보도적변화가이작위산다속식물속하적분류의거지일.저야암시료이용표준홍외광보도고,가이구분화감정출산다속식물적충류.경주성분분석후적홍외광보수거구건적수형취류도여선전적형태분류결과대체일치,능장유다조sect.Oleifera화단주다조sect.Paracamellia식물명현구분,병차각조중친연관계교근적충취재일기.인차지지타문작위량개독립적조처리.단시,홍산다조sect.Camellia내적전산다아조subsect.Lucidissima화광과홍산다아조subsect.Reticulata식물재취류도상흔난구분,건의장저량개아조식물진행귀병.최후토론료장굉체화민천록계통중존재분기적유다조、단주다조화홍산다조내적충간분류관계.
Sixty-five leaf samples in sect.Oleifera H.T.Chang,sect.Paracamellia Sealy.sect.Camellia,and sect.Thea(L.)Dyer of genus Camellia L.were discriminated directly with an OMNI-sampler accessory on the basis of biochemical profles and a hierarchical dendrogram was finally constructed.The results showed that the infrared spectra of Camellia were fingerprint-like patterns which were highly typical for different taxa.The hierarchical dendrogram based on principal component analysis of Fourier Transform Infrared(FTIR) data confirmed most of morphological classifications of the four sections proposed in previous works.Infrared spectra of leavesare of taxonomic value in genus Camellia,and this technique can be widely used for identification and classification of other taxa when standard spectra are available.The relationships between sect.Oleifera and sect.Paracamellia,subsect.Lucidissima H.T.Chang and subsect.Reticulata H.T.Chang in sect.Camellia,and the species/varieties were also discussed,as many dissensions about the classification exist between Chang's and Ming'ssystem.