CAJ | 학술논문

目的比较推算法和酶免法测定血清游离睾酮(FT)的结果,探寻FT诊断中老年男子部分性雄激素缺乏综合征(PADAM)的界限值,并评估游离睾酮指数(FTI).方法收集129例45岁以上健康男性的血清标本,酶标免疫法测定游离睾酮(aFT),同时测定总睾酮(T)和性激素结合球蛋白(SHBG)并代人Vermeulen公式推算游离睾酮(cFT),对两种方法的FT值进行比较.其中60例,根据视听性刺激(AVSS)时阴茎勃起临测结果分组,计算勃起正常组cFT的参考范围.以此值为依据,对FTI进行有效性检验.结果 1.cFT和FTI与年龄相关性较好,而cFT与aFT值相差有统计学意文.2.cFT:勃起正常组0.37(0.20～0.52)nmol/L,异常组0.25(0.12～0.40)nmol/L.3.FTI:cFT异常组0.15,正常组0.51.FTI敏感性97.78%,特异性58.33%.结论推算法测定血清FT较酶免法科学、准确.0.20nmol/L是cFT诊断PADAM的界限值.FTI可作为PADAM病人治疗反应的随访.
목적 비교추산법화매면법측정혈청유리고동(FT)적결과,탐심FT진단중노년남자부분성웅격소결핍종합정(PADAM)적계한치,병평고유리고동지수(FTI).방법 수집129례45세이상건강남성적혈청표본,매표면역법측정유리고동(aFT),동시측정총고동(T)화성격소결합구단백(SHBG)병대인Vermeulen공식추산유리고동(cFT),대량충방법적FT치진행비교.기중60례,근거시은성자격(AVSS)시음경발기림측결과분조,계산발기정상조cFT적삼고범위.이차치위의거,대FTI진행유효성검험.결과 1.cFT화FTI여년령상관성교호,이cFT여aFT치상차유통계학의문.2.cFT:발기정상조0.37(0.20～0.52)nmol/L,이상조0.25(0.12～0.40)nmol/L.3.FTI:cFT이상조0.15,정상조0.51.FTI민감성97.78%,특이성58.33%.결론 추산법측정혈청FT교매면법과학、준학.0.20nmol/L시cFT진단PADAM적계한치.FTI가작위PADAM병인치료반응적수방.
Objective To compare serum free testosterone (FT) level obtained by calculation method or direct measurement, define a threshold value of FT for the diagnosis of partial androgen deficiency in the aging male (PADAM) and critically evaluate free testosterone index (FTI) with this criterion. Methods FT levels obtained by direct enzyme linked immunosorbent assay (aFT) and by calculation with the Vermeulen's formula (cFT) after determining the total testosterone (T)and sex-hormone-binding globulin (SHBG) were compared in 129 healthy males over 45 years. Of them, 60 men were divided into normal or abnormal groups according to the results of the erection function in audio-visual sexual stimulation (AVSS) test,where the reference range of cFT natural level was calculated, and the validity of FTI with the threshold value was evaluated extensively. Results 1. Both cFT and FTI correlated well with age, while cFT values varied considerably with aFT; 2. The reference value of cFT in normal and abnormal erection groups was 0.37(0.20～0.52 percentile) nmol/L and 0.25(0.12～0.40percentile) nmol/L respectively; 3. Using the threshold value of cFT 0.20nmol/L as the criterion, FTI value was 0.15 in abnormal erection group and 0.51in normal group. The seusitivity of FTI was 97.78% and the specificity of FTI was 58.33%. Conclusions cFT seems to be more scientific and accurate than aFT. This study supports the use of cFT with the threshold value 0.20nmol/L as an acceptable parameter for diagnosing PADAM. FTI could be used as an index for after-treatment follow-up of patients with hypogonadism.