CAJ | 학술논문

背景:越来越多的研究事实表明免疫系统不是一个仅有自主调节的孤立系统,而是与中枢神经系统之间存在双向联系.目的:探讨哮喘大鼠肺脏和大脑中c-fos表达的分布及其意义.设计:随机对照实验.单位:南方医科大学南方医院肿瘤科和珠江医院神经外科.材料:实验在2004-01/08在南方医科大学南方医院肿瘤科实验室和珠江医院神经外科完成.选择成年健康雄性大鼠14只.随机分为实验组10只和对照组4只.方法:实验组第1天用卵蛋白10mg加氢氧化铝干粉200 mg与灭活百日咳菌苗5×109个配成2 mL混悬液腹腔注射,于第15天开始用10 g/L卵蛋白超声雾化吸入,2次/h,共3 d,制作卵蛋白致敏大鼠哮喘模型,对照组第1天用生理盐水2 mL腹腔注射,于第15天用生理盐水雾化吸入,30 mL/d,共3 d.所有大鼠在麻醉状态下灌流固定取肺和脑组织,采用免疫组织化学卵白素-生物素-过氧化物复合物法和图像分析等技术观察Fos蛋白在肺脏和大脑内的分布情况.主要观察指标:Fos蛋白在脑内不同区域和肺脏中的分布.结果:14只大鼠均进入结果分析.①哮喘组大鼠肺脏和大脑中c-fos表达明显高于对照组(P＜0.05); Fos阳性产物在脑内主要集中分布在额顶皮质、边缘前脑(扣带皮质、梨状皮质和中央杏仁核等)、丘脑室旁核、下丘脑室旁核、视上核、下丘脑外侧区、下丘脑室周核、孤束核、最后区和延髓腹外侧区内;小脑内无明显Fos分布密集区.②哮喘组气道壁及肺组织中可见大量的c-fos阳性细胞,阳性炎症细胞主要分布于黏膜层、黏膜下层及平滑肌周围;而对照组动物气道及肺组织中无或偶见免疫反应极弱的c-fos阳性细胞.结论:哮喘大鼠不仅肺脏中c-fos表达明显增加,而且在延髓内脏带及其上行投射神经核团(下丘脑、杏仁核等)表达也较多,说明原癌基因c-fos表达可能与哮喘神经免疫调节密切相关. 揹景:越來越多的研究事實錶明免疫繫統不是一箇僅有自主調節的孤立繫統,而是與中樞神經繫統之間存在雙嚮聯繫.目的:探討哮喘大鼠肺髒和大腦中c-fos錶達的分佈及其意義.設計:隨機對照實驗.單位:南方醫科大學南方醫院腫瘤科和珠江醫院神經外科.材料:實驗在2004-01/08在南方醫科大學南方醫院腫瘤科實驗室和珠江醫院神經外科完成.選擇成年健康雄性大鼠14隻.隨機分為實驗組10隻和對照組4隻.方法:實驗組第1天用卵蛋白10mg加氫氧化鋁榦粉200 mg與滅活百日咳菌苗5×109箇配成2 mL混懸液腹腔註射,于第15天開始用10 g/L卵蛋白超聲霧化吸入,2次/h,共3 d,製作卵蛋白緻敏大鼠哮喘模型,對照組第1天用生理鹽水2 mL腹腔註射,于第15天用生理鹽水霧化吸入,30 mL/d,共3 d.所有大鼠在痳醉狀態下灌流固定取肺和腦組織,採用免疫組織化學卵白素-生物素-過氧化物複閤物法和圖像分析等技術觀察Fos蛋白在肺髒和大腦內的分佈情況.主要觀察指標:Fos蛋白在腦內不同區域和肺髒中的分佈.結果:14隻大鼠均進入結果分析.①哮喘組大鼠肺髒和大腦中c-fos錶達明顯高于對照組(P＜0.05); Fos暘性產物在腦內主要集中分佈在額頂皮質、邊緣前腦(釦帶皮質、梨狀皮質和中央杏仁覈等)、丘腦室徬覈、下丘腦室徬覈、視上覈、下丘腦外側區、下丘腦室週覈、孤束覈、最後區和延髓腹外側區內;小腦內無明顯Fos分佈密集區.②哮喘組氣道壁及肺組織中可見大量的c-fos暘性細胞,暘性炎癥細胞主要分佈于黏膜層、黏膜下層及平滑肌週圍;而對照組動物氣道及肺組織中無或偶見免疫反應極弱的c-fos暘性細胞.結論:哮喘大鼠不僅肺髒中c-fos錶達明顯增加,而且在延髓內髒帶及其上行投射神經覈糰(下丘腦、杏仁覈等)錶達也較多,說明原癌基因c-fos錶達可能與哮喘神經免疫調節密切相關.
배경:월래월다적연구사실표명면역계통불시일개부유자주조절적고립계통,이시여중추신경계통지간존재쌍향련계.목적:탐토효천대서폐장화대뇌중c-fos표체적분포급기의의.설계:수궤대조실험.단위:남방의과대학남방의원종류과화주강의원신경외과.재료:실험재2004-01/08재남방의과대학남방의원종류과실험실화주강의원신경외과완성.선택성년건강웅성대서14지.수궤분위실험조10지화대조조4지.방법:실험조제1천용란단백10mg가경양화려간분200 mg여멸활백일해균묘5×109개배성2 mL혼현액복강주사,우제15천개시용10 g/L란단백초성무화흡입,2차/h,공3 d,제작란단백치민대서효천모형,대조조제1천용생리염수2 mL복강주사,우제15천용생리염수무화흡입,30 mL/d,공3 d.소유대서재마취상태하관류고정취폐화뇌조직,채용면역조직화학란백소-생물소-과양화물복합물법화도상분석등기술관찰Fos단백재폐장화대뇌내적분포정황.주요관찰지표:Fos단백재뇌내불동구역화폐장중적분포.결과:14지대서균진입결과분석.①효천조대서폐장화대뇌중c-fos표체명현고우대조조(P＜0.05); Fos양성산물재뇌내주요집중분포재액정피질、변연전뇌(구대피질、리상피질화중앙행인핵등)、구뇌실방핵、하구뇌실방핵、시상핵、하구뇌외측구、하구뇌실주핵、고속핵、최후구화연수복외측구내;소뇌내무명현Fos분포밀집구.②효천조기도벽급폐조직중가견대량적c-fos양성세포,양성염증세포주요분포우점막층、점막하층급평활기주위;이대조조동물기도급폐조직중무혹우견면역반응겁약적c-fos양성세포.결론:효천대서불부폐장중c-fos표체명현증가,이차재연수내장대급기상행투사신경핵단(하구뇌、행인핵등)표체야교다,설명원암기인c-fos표체가능여효천신경면역조절밀절상관.
BACKGROUND: Increasing evidence identifies the immune system not as an isolated system with automodulations, but one that interacts with the central nervous system.OBJECTIVE: To investigate the distribution of c-fos expression in the lung and brain tissues of asthmatic rats and explore is significance.DESIGN: Randomized controlled study.SETTING: Department of Oncology, Southern Hospital, and Department of Neurosurgery, Zhujiang Hospital, Southern Medical University.MATERIALS: The experiment was conducted Department of Oncology,Southern Hospital, and Department of Neurosurgery, Zhujiang Hospital,Southern Medical University, between January and August 2004. Fourteen healthy male rats were randomized into experimental group (n=10) and control group (n=4).METHODS: On the first day of experiment, the rats in experimental group received intraperitoneal injection of 2 mL of the suspension containing 10 mg albumen, 200 mg aluminum hydroxide powder and inactivated pertussis vaccine (5×109), and subjected to inhalation of ultrasonically atomized 10 g/L albumen from on the 15th day, 2 times per hour for totally 3 days, to induce asthma in the rats. The rats in the control group received intraperitoneal injection of 2 mL normal saline on the 1st day and inhalation of ultrasonically normal saline on the 15th day, 30 mL a day for totally 3 days. The lung and brain tissues of all the anesthetized rats were fixed by perfusion, and immunohistochemical method with ovin-biotin-peroxidase complex and imaging analysis system were used to observe the distribution of Fos protein in the lung and brain.MAIN OUTCOME MEASURES: Distribution of c-Fos protein in lung and cerebrum.c-Fos in the lung and brain tissues was obviously higher in asthmatic group than in the control group (P ＜ 0.05), located mainly in the parietal-fontal cortex, limbic forebrain (cingulum cortex, pyriform cortex and central amygdaloid nucleus and so on), thalamus paraventricular nucleus, hypothalamus paraventricular nucleus, supraoptic nucleus, lateral region of the hypothalamus, hypothalamus periventricular nucleus, nucleus of solitary tract,area postrema and ventrolateral medulla. No obvious Fos expression was observed in the cerebellum. A large number of c-Fos-positive cells were observed in the wall of the airway and lungs in asthmatic rats, mainly distributing in the mucous membrane and submucous layer and around the smooth muscles; in the control rats, no positive cells or only occasional cells with weak c-Fos positivity were found in the wall of the airway and lungs.CONCLUSION: c-Fos expression increases obviously not only in the lungs of asthmatic rats, but also in medullar and its ascending projecting nuclei (hypothalamus, amygdala and so on), suggesting that the expression of protooncogene c-fos might be closely related with neuroimmunomodulation in asthma.