CAJ | 학술논문

目的：了解长白山区莱姆病传播媒介-全沟硬蜱的地理位置、分布及其病原携带情况。方法：用酶联免疫吸附试验、间接免疫荧光试验检测莱姆病特异IgG抗体。结果：长白山区抓捕的620只硬蜱，全沟硬蜱为593只，占总数的95.80%，用BSK培养基33℃培养，分纯后共获得纯阳性伯氏疏螺旋体培养物243株，用兔抗美国B31特异性多克隆抗体和针对莱姆病螺旋体种属特异单克隆抗体，间接免疫荧光抗体试验进行鉴定，证明其单克隆抗体反应与美国菌株稍有差异，与国内分离株M7相同。血检826人，IgG、IgM≥1∶128(抗体滴度)有49例，阳性率为5.93%；IgG、IgM≥1∶64(抗体滴度)有82例；二项合计为15.85%。结论：长白山区是莱姆病重要疫源地，人群中有莱姆病感染存在，并有莱姆病发生。
목적：료해장백산구래모병전파매개-전구경비적지리위치、분포급기병원휴대정황。방법：용매련면역흡부시험、간접면역형광시험검측래모병특이IgG항체。결과：장백산구조포적620지경비，전구경비위593지，점총수적95.80%，용BSK배양기33℃배양，분순후공획득순양성백씨소라선체배양물243주，용토항미국B31특이성다극륭항체화침대래모병라선체충속특이단극륭항체，간접면역형광항체시험진행감정，증명기단극륭항체반응여미국균주초유차이，여국내분리주M7상동。혈검826인，IgG、IgM≥1∶128(항체적도)유49례，양성솔위5.93%；IgG、IgM≥1∶64(항체적도)유82례；이항합계위15.85%。결론：장백산구시래모병중요역원지，인군중유래모병감염존재，병유래모병발생。
Objective:To investigate the distribution of Ixodes persulcatusand the relation with Lyme disease.Methods:To detect the specific IgG by means of ELISA and indirect immunofluorescence.Results:We investigated on geographical conditions, distribution of Ixodes persulcatus and isolated Lyme disease spirochetes in Changbaishan. We conducted the survey of specific IgG by means of ELISA and indirect immunofluorescence and found that there were 593(95.80%) Ixodes persulcatus in 620 Ixodes ang got 243 positive Borrelia burgdorferi culture. By means of hemospasia survey the sera of 826 mountain residents were tested for antibodies, 131(15.85%) mountain residents had significant IgG titers, including 49(5.93%) IgG, IgM≥1∶128 and 82(9.82%) IgG,IgM≥1∶64. Conclusion:It was comfirmed that Changbaishan is an important epidemic focus. There are infections of Lyme disease among the mountain residents in Changbaishan.