CAJ | 학술논문

目的：初步建立血浆内毒素定量检测模式，提出健康人群血浆内毒素正常值及内毒素血症诊断参考值。方法：用稀释加热法排除血浆干扰，以弃G因子鲎试剂在BET-16细菌内毒素测定仪下检测被试者血浆内毒素水平，并将结果与临床病原微生物培养比较。结果：该法强化内毒素回收率50%～150%，符合美国FDA 1987年版要求。在临床感染91例患者中，内毒素血症37例，全部分离出＞1株的G-杆菌，其余54例血浆内毒素正常的患者中，只有5例分离出G-杆菌。结论：该法快速，简便，可作为G-杆菌感染及内毒素血症的诊断方法。
목적：초보건립혈장내독소정량검측모식，제출건강인군혈장내독소정상치급내독소혈증진단삼고치。방법：용희석가열법배제혈장간우，이기G인자후시제재BET-16세균내독소측정의하검측피시자혈장내독소수평，병장결과여림상병원미생물배양비교。결과：해법강화내독소회수솔50%～150%，부합미국FDA 1987년판요구。재림상감염91례환자중，내독소혈증37례，전부분리출＞1주적G-간균，기여54례혈장내독소정상적환자중，지유5례분리출G-간균。결론：해법쾌속，간편，가작위G-간균감염급내독소혈증적진단방법。
Objective:To establish a routine for quantitating endotoxin in blood plasma and determine the reference value of endotoxin in blood plasma of healthy subjects for diagnosis of infectious diseases.Method:The confounding influence of blood plasma was eliminated by dilution and heating. The level of endotoxin in plasma was measured with the BET-16 measuring equipment of bacterial endotoxin by using limulus lysate G factor and compared with to pathogenic microorganism results.Results:The assay can potentiate the endotoxin recovery rate by 50%-150%. Symboling FDA 1987. of the 91 cases examined,37 cases were with endotoxemia and more than 1 gram-negative bacteria isolates. The other 54 cases were with normal level of endotoxin in plasma,of whom only 5 cases were with gram negative bacteriaisolates.Conclusion:The assay was simple,sensitive and quantitative. The method was effective in the determination of gram negative bacterial infection and endotoxemia.