CAJ | 학술논문

本文采用离心分离的方法获得细胞质膜后，再用去污剂SDS(十二烷基磺酸钠)溶解质膜成分。将所得到的富质膜成分样品进行排阻高效液相色谱分离。用ICP-MS和ICP-AES分别检测级分溶液中的稀土元素含量和钙含量。研究结果表明，在根细胞质膜上有La，而且La至少有四种不同的结合形态，其他稀土元素Ce\,Pr和Nd同La一样也有类似的四种结合形态。
본문채용리심분리적방법획득세포질막후，재용거오제SDS(십이완기광산납)용해질막성분。장소득도적부질막성분양품진행배조고효액상색보분리。용ICP-MS화ICP-AES분별검측급분용액중적희토원소함량화개함량。연구결과표명，재근세포질막상유La，이차La지소유사충불동적결합형태，기타희토원소Ce\,Pr화Nd동La일양야유유사적사충결합형태。
Plasma membranes of cells of soybean roots were prepared by centrifugation method and solubilizated by SDS.Plasma membrane components-enriched sample was chromatographed on size exclution column.Rare earth element contents or calcium contents of fraction solutions were determined by ICP-MS or by ICP-AES.Experimental results showed that there was lanthanum in plasma membranes of cells of soybean roots and it had at least four speciations.Ce,Pr and Nd had same ones as La.