CAJ | 학술논문

以几种新型细胞质雄性不育型小麦为材料，对分蘖期小麦叶片中的依赖钙/钙调素的蛋白激酶(Ca2+/CaM-PK)的SDS-PAGE电泳图谱进行了分析，实验结果表明，不育系与保持系的蛋白激酶酶带之间存在差别，并且不育系之间也有差别，说明不育细胞质对核基因的表达也有影响,这可能与雄性不育有关,而且不育材料中存在潜在优势，可用来选育具有更强优势组合的核质杂种.
이궤충신형세포질웅성불육형소맥위재료，대분얼기소맥협편중적의뢰개/개조소적단백격매(Ca2+/CaM-PK)적SDS-PAGE전영도보진행료분석，실험결과표명，불육계여보지계적단백격매매대지간존재차별，병차불육계지간야유차별，설명불육세포질대핵기인적표체야유영향,저가능여웅성불육유관,이차불육재료중존재잠재우세，가용래선육구유경강우세조합적핵질잡충.
Using four types(C,G,M,Mt2) of cytoplasmic male sterile wheat as experiment materials, their maintainer Chinese spring wheat (T.aestivum) as contrast, the zymograms of Ca2+/CaM-dependent protein kinase were studied. The electrophoresis were carried out after crude enzyme of Ca2+/CaM-PK were extracted from leaves in tillering stage. The bands were well separated upon linear gradient SDS-PAGE(6%-15％).There existed difference among male sterile materials, maintainer and Huamai No.8. So the cytoplasm have different effect on the expression of gene, which probably associated with the cause of male sterility.The new male sterile wheat may be used to create new hybrid.