生殖与避孕(英文版)
生殖與避孕(英文版)
생식여피잉(영문판)
REPRODUCTION & CONTRACEPTION
2001年
3期
140-146
,共7页
吴小芳%陈晖%费仁仁%陈松%曹坚
吳小芳%陳暉%費仁仁%陳鬆%曹堅
오소방%진휘%비인인%진송%조견
Objective To investigate the existence of the protamine Mrna in sperms of human,rat and mouse Methods By means of RT-PCR technique, protamine cDNA fragments were obtained from total RNA of the mature sperms of human, rat and mouse respectively.Results mRNA of protamine gene was found in the mature sperm of human, rat and mouse. The protamine cDNA with an abnormal head obtained by PT-TCR in rat sperm was much less tn number than that in the normal rat sperm.Conclusion mRNA in the sperms might represent the condition of corresponding gene expression during spermatogenesis.
