CAJ | 학술논문

在饲料中添加1‰的虫草多糖，以口服形式对日本沼虾进行免疫。通过连续测定日本沼虾血淋巴吞噬活性，血清中抗菌活力、溶菌活力以及酚氧化酶活力，研究虫草多糖对日本沼虾免疫机能的作用。结果表明：除个别情况外，虫草组的吞噬百分比和吞噬指数、抗菌活力、溶菌活力以及酚氧化酶活力，均高于对照组。因此,投喂虫草多糖能够明显地增强日本沼虾的免疫防御能力。
재사료중첨가1‰적충초다당，이구복형식대일본소하진행면역。통과련속측정일본소하혈림파탄서활성，혈청중항균활력、용균활력이급분양화매활력，연구충초다당대일본소하면역궤능적작용。결과표명：제개별정황외，충초조적탄서백분비화탄서지수、항균활력、용균활력이급분양화매활력，균고우대조조。인차,투위충초다당능구명현지증강일본소하적면역방어능력。
Cordyceps polysaccharide, a product bought from med icine shops, with th e characteristics similar to those of β-glucan, was used as a food additive to test its immune effect. Macrobrachbium nipponense with a body we ight of 2.0～2.5 g were collected from the Dadongmen Aquaculture Market on Sept em ber 12, 2000，and nursed. Water temperature varied from 29℃　to　14℃ （n atural temperature），the water was aerated and changed once a day（1/2～2/3 volume of water was renewed each time）. 　　The experiment of phenoloxidase （PO） test was designed as the same as those of bacteriolytic（Ul）and antibacterial （Ua）activity tests. Blood samples w ere c ollected from the haemocoel of 10～15 shrimps to measure PO, Ul, Ua activity on 0, 1, 4, 7, 14, 21 day. The phagocytosis was still measured after stopping the polysaccharide's feeding. 　　The results showed that PO, Ua, Ul and phagocytosis of the test group are si gnif icantly higher than those of the controll group, indicating the effectiveness of cordceps polysaccharide on enhancing PO, Ua, Ul and phagocytosis of M. ni pponen se，leading to an increase in the immunnological activities. Further studies a re needed to identify the mechanism of cordceps polysaccharide in immune effect.