重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2001年
2期
100-101
,共2页
聚合酶链反应%乙型肝炎病毒%前S2蛋白抗原
聚閤酶鏈反應%乙型肝炎病毒%前S2蛋白抗原
취합매련반응%을형간염병독%전S2단백항원
目的 探讨乙型肝炎病毒(HBV)DNA与六种常见HBV血清免疫学标志物模式、前S2蛋白抗原(Pre-S2)之间的相互关系及其临床检测意义。方法 采用PCR法对HBV DNA、ELISA法对HBV血清免疫学标志物、Pre-S2同时进行检测。结果 1 484例六种常见模式HBV DNA阳性率依次为89.8%>55.6%>21.8%>7.5%>7.1%>6.8%,即模式(1)>(3)>(2)>(5)>(6)>(4);而HBV血清免疫学标志阳性例数依次为(2)>(1)>(3)>(5)>(4)>(6)。(2)、(3)种模式HBV DNA与Pre-S2阳性率比较P<0.01,其余(1)、(4)~(6)种模式HBV DNA与Pre-S2阳性检出率无显著性差异(P>0.05)。结论 HBV血清免疫学标志物、Pre-S2、HBV DNA的检测,三者缺一不可,ELISA法检测HBV血清免疫学标志物、Pre-S2,只是HBV的表型指标,提供HBV感染的间接证据。而PCR-HBV DNA的检测是HBV感染与否的直接证据。因此它们各自有其独特的临床检测意义。
目的 探討乙型肝炎病毒(HBV)DNA與六種常見HBV血清免疫學標誌物模式、前S2蛋白抗原(Pre-S2)之間的相互關繫及其臨床檢測意義。方法 採用PCR法對HBV DNA、ELISA法對HBV血清免疫學標誌物、Pre-S2同時進行檢測。結果 1 484例六種常見模式HBV DNA暘性率依次為89.8%>55.6%>21.8%>7.5%>7.1%>6.8%,即模式(1)>(3)>(2)>(5)>(6)>(4);而HBV血清免疫學標誌暘性例數依次為(2)>(1)>(3)>(5)>(4)>(6)。(2)、(3)種模式HBV DNA與Pre-S2暘性率比較P<0.01,其餘(1)、(4)~(6)種模式HBV DNA與Pre-S2暘性檢齣率無顯著性差異(P>0.05)。結論 HBV血清免疫學標誌物、Pre-S2、HBV DNA的檢測,三者缺一不可,ELISA法檢測HBV血清免疫學標誌物、Pre-S2,隻是HBV的錶型指標,提供HBV感染的間接證據。而PCR-HBV DNA的檢測是HBV感染與否的直接證據。因此它們各自有其獨特的臨床檢測意義。
목적 탐토을형간염병독(HBV)DNA여륙충상견HBV혈청면역학표지물모식、전S2단백항원(Pre-S2)지간적상호관계급기림상검측의의。방법 채용PCR법대HBV DNA、ELISA법대HBV혈청면역학표지물、Pre-S2동시진행검측。결과 1 484례륙충상견모식HBV DNA양성솔의차위89.8%>55.6%>21.8%>7.5%>7.1%>6.8%,즉모식(1)>(3)>(2)>(5)>(6)>(4);이HBV혈청면역학표지양성례수의차위(2)>(1)>(3)>(5)>(4)>(6)。(2)、(3)충모식HBV DNA여Pre-S2양성솔비교P<0.01,기여(1)、(4)~(6)충모식HBV DNA여Pre-S2양성검출솔무현저성차이(P>0.05)。결론 HBV혈청면역학표지물、Pre-S2、HBV DNA적검측,삼자결일불가,ELISA법검측HBV혈청면역학표지물、Pre-S2,지시HBV적표형지표,제공HBV감염적간접증거。이PCR-HBV DNA적검측시HBV감염여부적직접증거。인차타문각자유기독특적림상검측의의。
Objective To investigate the clinical significance and relationship among Pre-S2、6 common types of HBV serum immune markers(HBV-M)and HBV-DNA.Methods Using PCR to detect HBV DNA and ELISA technique to detect Pre-S2,6 kinds of HBV-M in the meantime.Results Positive infection rates of HBV DNA in 6 common types of the 1484 cases were 89.8%>55.6%>21.8%>7.5%>7.1%>6.8% respectively,namely the model (1)>(3)>(2)>(5)>(6)>(4);but the positive cases of HBV serum immune markers were in the order (2)>(1)>(3)>(4)>(5)>(6).The positive rates were significant difference between Pre-S2 and HBV DNA in (2)、(3) types (P<0.01) and no significant difference in other types(P>0.05).Conclusion It is necessary to detect Pre-S2,HBV DNA and HBVserum immune markers,but HBV serum immune makers and Pre-S2 detected by ELISA only external indication and indirect evidence of HBV infection.While the detection of HBV DNA by PCR was direct evidence of HBV infection.Therefore they have their unique clinicl significance.