CAJ | 학술논문

以籼稻品种IR36为材料，采用塑料半薄切片技术、新鲜花药整体观察及游离的雄性细胞观察方法，对水稻花粉发育全过程及其药壁组织的情况进行了系统的研究，初步将这一过程划分为8个时期，即小孢子母细胞形成期、小孢子母细胞减数分裂期、小孢子早期、小孢子中期、小孢子晚期、二胞花粉早期、二胞花粉晚期和成熟花粉期。并证实了花粉自然发育过程中不存在收缩期。在这一发育过程中，绒毡层出现3种明显不同的发育形态，这些形态特点对于判断花粉发育阶段具有重要的参考价值及辅助作用。
이선도품충IR36위재료，채용소료반박절편기술、신선화약정체관찰급유리적웅성세포관찰방법，대수도화분발육전과정급기약벽조직적정황진행료계통적연구，초보장저일과정화분위8개시기，즉소포자모세포형성기、소포자모세포감수분렬기、소포자조기、소포자중기、소포자만기、이포화분조기、이포화분만기화성숙화분기。병증실료화분자연발육과정중불존재수축기。재저일발육과정중，융전층출현3충명현불동적발육형태，저사형태특점대우판단화분발육계단구유중요적삼고개치급보조작용。
By using techniques of intact fresh anther observation,isolated male cell observation and semi-thin section,an extensively cytological study was conducted on the pollen development of IR36,an indica cultivar.The results showed that the wholly developmental process could be tentatively divided into eight stages,i.e.microspore mother cell formation stage,microspore mother cell meiosis stage,early microspore stage,middle microspore stage,late microspore stage,early bicellular pollen stage,late bicellular pollen stage and mature pollen stage.The experiment proved that there was not contraction stage in naturally developmental process of the microspores.During the pollen developing,the tapetal ceils underwent three types of morphological changes,which help to identify the stages of pollen development.