CAJ | 학술논문

目的了解尿激酶型纤溶酶原激活物u-PA及其抑制因子PAI-1在各类肾小球病变中的表达及其意义。方法采用免疫组化方法检测了120例人肾穿活检标本中各型肾炎的u-PA、PAI-1、Ⅳ型胶原的表达水平，以及肾小球内PCNA阳性细胞核数，用图像分析方法对其u-PA和PAI-1进行光密度定量。结果各型肾小球肾炎中u-PA和PAI-1均有不同程度的表达，与轻微病变组相比均有显著性差异。但各组中两者表达强度有所不同，PAl-l的阳性强度基本上高于同组的u-PA，两者差异显著(P＜0.05)。u-PA的表达增强与肾小球Ⅳ型胶原的积聚和PCNA阳性细胞核数的增多明显相关(r＝0.761、r＝0.811，P＜0.05)；而PAI-1的表达则主要与肾小球内Ⅳ型胶原增多相关(r=0 857，P＜0.05)。结论 u-PA和PAI-1的异常表达是影响肾小球肾炎发生发展的重要因素。
목적 료해뇨격매형섬용매원격활물u-PA급기억제인자PAI-1재각류신소구병변중적표체급기의의。방법 채용면역조화방법검측료120례인신천활검표본중각형신염적u-PA、PAI-1、Ⅳ형효원적표체수평，이급신소구내PCNA양성세포핵수，용도상분석방법대기u-PA화PAI-1진행광밀도정량。결과 각형신소구신염중u-PA화PAI-1균유불동정도적표체，여경미병변조상비균유현저성차이。단각조중량자표체강도유소불동，PAl-l적양성강도기본상고우동조적u-PA，량자차이현저(P＜0.05)。u-PA적표체증강여신소구Ⅳ형효원적적취화PCNA양성세포핵수적증다명현상관(r＝0.761、r＝0.811，P＜0.05)；이PAI-1적표체칙주요여신소구내Ⅳ형효원증다상관(r=0 857，P＜0.05)。결론 u-PA화PAI-1적이상표체시영향신소구신염발생발전적중요인소。
Purpose To investigate the significance of u-PA and PAI-1 expression in various types ofglomerulonephritis. Methods The expression level of u-PA, PAI-1, type-Ⅳ collagen and PCNA positivenuclei in 120 cases of renal biopsies from patients with various types of glomerulonephritis were detected byimmunohistochemical method. Both the staining intensity of u-PA and PAI-1 in glomeruli were quantitativelyanalyzed by image analysis. Results The expression intensity of u-PA and PAI-1 was different in varoustypes of glomerulonephritis, which were significantly higher than that of the minor lesion group. Meanwhile,the intensity of PAI-1 stain was significantly higher than that of u-PA in various types of glomerulonephritis( P ＜ 0.05). The increase of u-PA expression was closely related to increase of type- Ⅳ collagen synthesis andhypercellularity in glomeruli(r = 0. 761 and 0. 811, P＜ 0.05), while the expression of PAI-1 was closelyrelated to the increase of Col-Ⅳ synthesis other than the cell proliferation in the glomeruli. ConclusionsThe over expression of u-PA and PAI-1 may play an important role in contributing to the pathogenesis anddevelopment of glomerulonephritis.