CAJ | 학술논문

建立了尿中三唑仑的代谢物α羟基三唑仑和阿普唑仑的代谢物α羟基阿普唑仑的气相色谱电子捕获检测法。代谢物用N，O双三甲基硅烷基三氟乙酰胺（BSTFA）进行三甲基硅烷衍生化后进行检测。尿中代谢物的检出限约为1μg/L（S/N=3），至少可检出口服治疗量药物人体24h之内排泄尿中的代谢物，满足麻醉后犯罪案件中药物检验的要求。
건립료뇨중삼서륜적대사물α간기삼서륜화아보서 륜적대사물α간기아보서륜적기상색보전자포획검측법。대사물용N，O쌍삼갑기규완기삼불을선알（BSTFA）진행삼갑기규완연생화후진행검측。뇨중대사물적검출한약위1μg/L（S/N=3），지소가검출구복치료량약물인체24h지내배설뇨중적대사물，만족마취후범죄안건중약물검험적요구。
A method was developed to assay α-Hydroxyltriazolam and α-Hydroxyalprazolam,　which are the major metabolites of triazolam and alprazolam respectively,in human urine.　After addition of 2-hydroxyflurazepam　(interal standard) and hydrolysis with β-glucuronidase, the hydroxy-metabolites were extracted with hexane-dichloromethane (1∶1) at pH　10.8, then were derivated with (BSTFA). The analysis was performed on a HP-5 capillary column with electron-capture detector.The detection limits of analysis in urine were about 1μg/L.The method was successfully applied to urine specimens collected from healthy human volunteers who ingested 0.5 mg of triazolam or 0.8 mg alprazolam. The method was enough sensitive to assay urine specimen excreted at 24 h by volunteers after taking the medicine.